Evaluation of Antimicrobial Pattern of Difficult-To-Treat Resistant Gram-negative Bacteria from Pus Samples in a Tertiary Care Hospital

Warda Furqan; Faisal Hanif; Javaid  Usman; Rafia Irfan; Afnan Naeem

doi:10.51253/pafmj.v76i3.12617

Authors

Warda Furqan Department of Microbiology, Army Medical College, Rawalpindi/National University of Medical Sciences (NUMS) Pakistan
Faisal Hanif Department of Microbiology, Quetta Institute of Medical Sciences/National University of Medical Sciences (NUMS) Pakistan
Javaid Usman Department of Microbiology, Army Medical College, Rawalpindi/National University of Medical Sciences (NUMS) Pakistan
Rafia Irfan Department of Microbiology, Army Medical College, Rawalpindi/National University of Medical Sciences (NUMS) Pakistan
Afnan Naeem Department of Microbiology, Army Medical College, Rawalpindi/National University of Medical Sciences (NUMS) Pakistan

DOI:

https://doi.org/10.51253/pafmj.v76i3.12617

Keywords:

Antibiotic Resistance, Antibiotic Pattern, Bacterial Infection, Gram-negative Bacteria, Pyogenic.

Abstract

Objective: To assess the antimicrobial profile of difficult-to-treat resistant Gram-negative bacteria, isolated from pus specimens at a tertiary care hospital.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Microbiology, Pak Emirates Military Hospital and Army Medical College (NUMS) Rawalpindi, Pakistan, from Jun to Aug 2024.

Methodology: A total of 1,250 pus specimens were collected. Pus was processed according to standard procedure of microbiology and antibiotic susceptibility testing was done following Clinical and Laboratory Standards Institute 2023 protocols. Analysis of data was done using Statistical Package for Social Sciences version (SPSS) version 26. Descriptive factors including frequencies and percentage were calculated. Chi square test was applied to check for association. A p-value of less than or equal to 0.05 was assumed significant.

Results: Out of 1,250 pus specimens received during one year, 416(33.2%) were Gram-negative rods and 356(28.4%) were Gram-positive cocci. The most frequently isolated organism was Escherichia coli, seen in 156(30.3%) samples, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. Acinetobacter baumannii appeared as the most resistant pathogen. It was sensitive to only colistin and tigecycline.

Conclusion: By determining various types of Gram-negative rods present in pus specimens, as well as their sensitivity for different antibiotics an institutional antibiogram can be constructed. It will guide clinicians in making valuable treatment decisions and indirectly help preventing antimicrobial resistance and promote optimal patient care.

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