Expression of Genetic Polymorphism of TMPRSS2 Gene (Rs12329760) in Different Ethnicities of Prostate Cancer Patients: A Case Control Study

Saadia Sadiq; Amena Rahim; Muhammad Afzal; Sahar Ikram; Tehseen Ashraf Kohistani; Farzana Munir

doi:10.51253/pafmj.v75iSUPPL-7.9608

Authors

Saadia Sadiq Department of Biochemistry, Abbottabad International Medial College Abbottabad, Pakistan
Amena Rahim Department of Biochemistry, Islamic International Medical College, Rawalpindi, Pakistan
Muhammad Afzal Department of Biochemistry, Islamic International Medical College, Rawalpindi, Pakistan
Sahar Ikram Department of Biochemistry, Federal Medical and Dental College Islamabad Pakistan
Tehseen Ashraf Kohistani Department of Medicine, Combined Military Hospital, Bhawalpur/National University of Medical Sciences (NUMS) Pakistan
Farzana Munir Department of Pharmacology, Islamic International Medical College, Rawalpindi, Pakistan

DOI:

https://doi.org/10.51253/pafmj.v75iSUPPL-7.9608

Keywords:

Prostate Cancer, Single Nucleotide Polymorphism, TMPRSS2 Gene Polymorphism (rs12329760).

Abstract

Objective: To determine the frequency and clinical relevance of the TMPRSS2 gene polymorphism (rs12329760) in prostate cancer patients of different ethnicities.

Study Design: Case-control study.

Place and Duration of Study: Department of Biochemistry, Islamic International Medical College, Rawalpindi, Surgical unit of Railway General Hospital, Rawalpindi, and Urology Department of National Institute of Rehabilitative Medicine, Islamabad, Pakistan, from Oct 21 to Sep 22.

Methodology: There were 200 participants included in this study, with 100 cases of prostate cancer and 100 age-matched controls. Blood samples were collected from the outpatient department (OPD) and ward. The Chelex method was used for DNA extraction. Tetra-ARMS Polymerase chain reaction was carried out to determine the respective allelic frequencies of the TMPRSS2 gene polymorphism (rs12329760) using specific primers.

Results: CT and TT genotypes of TMPRSS2 gene polymorphism (rs12329760) showed a significant association in prostate cancer patients (p-value˂0.001). T-allele of TMPRSS2 gene polymorphism (rs12329760) also showed a significant association in prostate cancer patients (p-value<0.001). Significant association was also found in Pathans with CT and TT genotype of TMPRSS2 gene polymorphism (rs12329760) with p-values of 0.01 and 0.004. Punjabis also showed a significant association with CT genotype of TMPRSS2 gene polymorphism (rs12329760) with a p-value of 0.004.

Conclusion: TMPRSS2 gene polymorphism (rs12329760) is strongly linked to a higher risk of developing prostate cancer in Pathans and Punjabis within the Pakistani population.

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