Detection of Cytomegalovirus in Post Hematopoietic Stem Cells Transplant Recipients by Real-Time PCR

Saifullah Khan Niazi; Nadia Gulzar; Eijaz Ghani; faraz ahmed; Muhammad Ali Rathore; Noman Shakoor

doi:10.51253/pafmj.v73i5.8812

Authors

Saifullah Khan Niazi Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Nadia Gulzar Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Eijaz Ghani Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
faraz ahmed Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Muhammad Ali Rathore Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Noman Shakoor Department of Pathology, Armed forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v73i5.8812

Keywords:

Cytomegalovirus, Hematopoietic stem cell transplant, Real-time polymerase chain reaction

Abstract

Objective: To evaluate the frequency of Cytomegalovirus in Post Hematopoietic Stem Cell Transplant Recipients by molecular method.

Study Design: Cross-sectional study.

Place and Duration of Study: Virology Department, Armed Forces Institute of Pathology, Rawalpindi Pakistan Jan to Dec
2021.

Methodology: One hundred and twenty-six hematopoietic stem cell transplant (HSCT) recipients were included in this study and were separated into various age groups. All included patients were CMV IgG positive. Real-time polymerase chain
reaction (PCR) was performed on post-HSCT recipients to detect CMV.

Results: Out of 126 post-HSCT recipients, CMV was detected in 80(63%) patients and was not found in 46(36%) patients.
Among positive patients, 60(75%) were males, and 20(15%) were female. Maximum cases of CMV detection in post-HSCT
were found in 23 patients aged 1-10 years.

Conclusion: A high frequency of CMV in post-HSCT recipients was observed in this study. CMV cause major complications in immunocompromised patients, such as in transplant recipients. Prevention of CMV infection is a main part of post-transplant monitoring and management.