ASSOCIATION OF MAGNESIUM LEVELS WITH LIPID PROFILE AND INFLAMMATORY MARKERS IN THIAZIDE INDUCED HYPOMAGNESEMIC RATS

Abstract

Objective: To study the effect of Magnesium supplementation on lipid profile (TC, HDL, LDL, TG, VLDL) and inflammatory markers (CRP s-VCAM 1) in thiazide administered rats.
Study Design: Randomized control trial.
Place and Duration of Study: Department of Biochemistry & Molecular Biology, Army Medical College Rawalpindi from Feb 2010 to Feb 2011.
Material and Methods: Ninety male Sprague Dawley rats, of age 90 to 120 days, divided into three groups: Control, Hydrochlorothiazide (HCTZ) administered control and Hydrochlorothiazide + Magnesium Oxide (HTCZ + Mg2+), administered experimental groups. Levels of inflammatory markers, Magnesium, lipid profile were measured in blood, after three weeks.
Results: Mean values in control group were: Serum TG 104.39 mg/dl,TC151.86 mg/dl, HDL76.91 mg/dl, LDL 52.52 mg/dl, VLDL 19.78mg/dl, CRP 1784.27 mg/L, sVCAM-1 564.33ng/ml and serum Magnesium0.85mmol/l. Mean lipid profile values in HCTZ administered control group were Serum TG 150.04 mg/dl, TC 182.92 mg/dl, HDL 71.77 mg/dl, LDL 76.40 mg/dl,VLDL 32.56 mg/dl, CRP 2923.33 mg/L, sVCAM-1 2003.00 ng/ml and serum Magnesium 0.49 mmol/l. Mean lipid profile values in HCTZ + Magnesium administered experimental group were Serum TG 131.69 mg/dl,TC 157.60 mg/dl, HDL 73.55 mg/dl,LDL 58.2 mg/dl,VLDL 25.05mg/dl, CRP 2149.53 mg/L ,sVCAM-1 1516.60 ng/mland serum Magnesium 0.68mmol/l.
Conclusion: Levels of sVCAM-1, CRP, TG, TC, LDL and VLDL are increased in HCTZ administered group, with negative correlation with serum magnesium levels. Levels of these parameters decreased in HTCZ + Mg2+ administered group, showing that magnesium supplementation helped in normalizing derangements (p<0.05).