EFFECT OF ALPHA TOCOPHEROL ON CHRONIC STRESS INDUCED DERANGEMENTS INN NEUROPEPTIDEY ON SPRAGUE DAWLEY RATS

Abstract

Objective: To determine the chronic stress induced decline in plasma Neuropeptide Y (NPY) level and protective effects of Alpha Tocopherol (AT) on maintenance of Neuropeptide Y level.

Study Design: Quasi-experimental study.

Place and Duration of Study: The study settings were Al-Nafees Medical College and animal center of National Institute of Health Islamabad (NIH). Duration of research was one year from May 2015 to May 2016.

Methodology: After taking approval of institutional review board 45 male Sprague Dawley rats with inclusion criteria were chosen and allocated to three groups comprised of n=15. Group I was control, group II restraint stress and group III (restraint stress with supplementation of alpha-tocopherol). After 28 days of restraining stress induction plasma Neuropeptide Y level was determined by sandwich Enzyme Linked Immunosorbent Assay (ELISA).

Results: There was a statistically significant decline (p<0.05) in plasma Neuropeptide Y levels in restraint group as compared to control group and with alpha-tocopherol supplementation group.

Conclusion: Chronic restraint stress induction led to decline in Neuropeptide Y levels and alpha-tocopherol prevented fall in Neuropeptide Y levels.