TO EVUALATE THE AMELIORATIVE ROLE OF VITAMIN E ON NEPHROTOXICITY INDUCED BY VANCOMYCIN IN RABBITS

Abstract

Objective: To analyze the ameliorative effects of vitamin E on vancomycin-induced nephrotoxicity in rabbits.

Study Design: Laboratory based experimental study.

Place and Duration of Study: National Institute of Health, Islamabad and Department of Pharmacology and Therapeutics, Army Medical College, Rawalpindi, from Jul to Aug 2017.

Methodology: Study included seventy adult, male and female, rabbits weighing 1-2 kg. Group A served as a control group (n=10), group B (n=30) received vancomycin, 200mg/kg I/p dissolved in normal saline twice a day for seven days while group C (n=30) received oral vitamin E, 500mg/kg 5 days prior to vancomycin administration and for seven days after 200mg/kgI/p vancomycin administration. On the eighth day animals were sacrificed. Blood samples were drawn on day 0 and day 8. Estimation of kidney damage was assessed via serum urea, creatinine, sodium and potassium levels. Kidneys were assessed for histopathological examination.

Results: Vitamin E administration was buoyant in alleviating marked nephrotoxicity imposed by vancomycin (group B) by significantly decreasing serum urea (p<0.001), creatinine, (p<0.001), serum sodium (p<0.001) and serum potassium levels (p<0.001). Improvement in the renal morphology was also witnessed in group C.

Conclusion: Vitamin E successfully ameliorated the nephrotoxicity induced by vancomycin administration. This implicates the therapeutic importance of vitamin E as a nephroprotective agent.