EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS

Authors

  • Sajid Ali Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Muhammad Alamgir Khan Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Amina Rasul Watim Medical College Rawalpindi Pakistan
  • nadia Latif Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Kamil Asghar Imam Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Sumayya Bashir Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan

Keywords:

Cisplatin, Hepatotoxicity, Oxidative stress

Abstract

Objective: To study the effect of cisplatin administration on oxidative stress induced hepatotoxicity in male Sprague Dawley rats.

Study Design: Randomized controlled trial.

Place and Duration of Study: Study was conducted at department of Physiology, Army Medical College, Rawalpindi. Duration of study was 18 months from Oct 2014 to Apr 2016.

Material and Methods: The trial was performed on sixty male Sprague Dawley rats which were distributed randomly into two groups of 30 rats each. Group I received placebo whereas group II received intraperitoneal cisplatin 2mg/kg body weight two times a week for the period of 4 weeks. After successful treatment, animals were sacrificed and terminal blood sample was collected and used for estimation of serum AST, ALT, albumin
and 8-isoprostane. Dissection of rats were done and liver tissue sampled. Tissue homogenate was prepared from liver sample which was used for estimation of total glutathione levels.

Results: In group I, 8-isoprostane was 18.31 ± 3.35 pg/ml, total glutathione was 4.29 ± 0.42 μmol/L, ALT was 36.93 ± 4.72 IU/L, AST was 124.2 ± 12.75 IU/L and Albumin was 4.11 ± 0.26 g/dl whereas in group II, 8-isoprostane was 67.9 ± 8.14 pg/ml, total glutathione was 1.92 ± 0.28 μmol/L, ALT was 87.17 ± 6.47 IU/L, AST was 357.7 ± 19.37 IU/L and Albumin was 2.12 ± 0.25 g/dl. Levels of serum 8-isoprostane, ALT and AST were significantly raised (p<0.001) whereas serum albumin and total glutathione in liver tissue were found significantly low (p<0.001) in group II as compared to group I.

Conclusion: Cisplatin treatment causes hepatotoxicity by increased production of reactive oxygen species in male Sprague Dawley rats.

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Published

25-06-2019

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Original Articles

How to Cite

1.
Ali S, Khan MA, Rasul A, Latif nadia, Imam KA, Bashir S. EVALUATION OF OXIDATIVE STRESS INDUCED HEPATOTOXICITY PRODUCED BY CISPLATIN IN MALE SPRAGUE DAWLEY RATS. Pak Armed Forces Med J [Internet]. 2019 Jun. 25 [cited 2024 Jun. 17];69(3):500-04. Available from: https://pafmj.org/PAFMJ/article/view/3016