COMPARISON OF ANTIHYPERLIPIDAEMIC ACTIVITY OF EUGENIA JAMBOLANA FRUIT WITH PUNICA GRANATUM FRUIT IN DIET INDUCED HYPERLIPIDAEMIC RATS

Abstract

Objective: To compare the antihyperlipidemic effects of Eugenia Jambolana fruit pulp with Punica Granatum fruit in diet induced hyperlipidaemic rats at the same dose level.
Methods: An experimental randomized control study was conducted on seventy five male albino rats over a period of 14 weeks in University of Health Sciences Lahore. They were divided into five groups labelled A, B, C, D and E with fifteen rats in each group. Group A was kept as normal control, groups B, C, D and E were given hyperlipidaemic diet for six weeks. In group B no further intervention was done, group C and D were given
ethanolic extract of Eugenia Jambolana and Punica Granatum respectively for eight weeks. Group E was given combination of both for same duration. Serum total cholesterol (TC), high density lipoprotein cholesterol (HDLc), lowdensity lipoprotein cholesterol (LDL-c) and triglycerides (TG) were measured at zero, six and fourteen weeks.
Results: At fourteenth week significant reductions in TC, LDL-c and TG and a rise in HDL-c was observed in interventional groups C, D and E as compared to experimental hyperlipidaemic control group B (p <0.05). There was no significant difference at baseline (zero weeks) serum TC, HDL-c, LDL-c and TG of groups A, B, C, D and E; p > 0.57, p > 0.22, p > 0.56, p > 0.76, respectively. On sixth week, there was no significant difference between
groups B, C, D and E (p > 0.05). However, 15 rats of group A had significant lower levels of cholesterol, high density lipoproteins, low density lipoproteins and triglycerides when compared to 60 rats of groups B, C, D and E (p<0.05).
Conclusion: In male albino rats combination of ethanolic extracts of Eugenia Jambolana and Punica Granatum fruit pulps was most effective in lowering serum total cholesterol and triglycerides while decrease in low density lipoprotein cholesterol and rise in high density lipoprotein cholesterol was same as the extracts given alone.