ANTIBACTERIAL ACTIVITY OF METHYLGLYOXAL AGAINST MULTI-DRUG RESISTANT SALMONELLA TYPHI

Multi-Drug Resistant Salmonella Typhi

Abstract

Objective: To evaluate the antibacterial activity of MGO against MDR Salmonella typhi isolated from blood
culture specimens and compare this activity against non-MDR S. typhi and with other gram negative rods.
Study Design: Experimental study.
Place and Duration of Study: Department of Microbiology, University of Health Sciences Lahore, from Jul 2011 to
Jun 2012.
Material and Methods: A total of 157 isolates of S. typhi were collected from different hospitals of Lahore and
kept stored at -80°C. Morphological, biochemical and serological identification and antibiotic susceptibility
testing of the isolates was carried out as per CLSI 2011 guidelines. Agar dilution method was used for the
determination of MICs of MGO, using a multi-point inoculator. The data was compiled and results were
determined using SPSS version 17.
Results: Ninety-seven out of 157 isolates (61.8%) were MDR S. Typhi, while 60 (38.2%) were non-MDR S. Typhi.
MIC90 of MGO against MDR S. Typhi isolates was (0.20 mg/mL; 2.8 mM), against non-MDR S. Typhi and Gram
negative rods each, it was (0.21 mg/mL; 3.0 mM). When MICs of MGO against MDR S. Typhi group were
compared to those of non-MDR S. Typhi group, the p-value was 0.827 (p>0.05; statistically insignificant). Whereas,
the p-value of MICs of MGO against MDR S. Typhi group was 0.023 (p<0.05; statistically significant) when
compared to gram negative rods group.
Conclusion: MGO has good antibacterial activity against MDR and non-MDR S. Typhi, and other genera of Gram
negative rods.