Hepatoprotective Effects of Silymarin and Walnut Leaf Extract in Isoniazid and Rifampicin Induced Hepatotoxicity
DOI:
https://doi.org/10.51253/pafmj.v76i1.12233Keywords:
Drug-Induced Liver Injury, Isoniazid, Rifampicin, Silymarin, TuberculosisAbstract
Objective: To evaluate the hepatoprotective effects of Silymarin and walnut leaf extract in isoniazid-rifampicin induced hepatotoxicity in Sprague-Dawley rats.
Study Design: Laboratory-based experimental study.
Place and Duration of Study: Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad Pakistan, from Jun to Jul 2023.
Methodology: A total of 50 rats were divided in 5 groups containing 10 rats each. Group-A was control group, in Group-B hepatotoxicity was induced by isoniazid 50mg/kg/day and Rifampicin 100 mg/kg/day co-administration daily for 14 days, Group-C was given isoniazid 50mg/kg/day and rifampicin 100 mg/kg/day and 200 mg/kg/day silymarin for 14 days , Group-D was given isoniazid 50mg/kg/day and rifampicin 100 mg/kg/and walnut leaf extract was administered at a dosage of 0.2 g/kg one hour before isoniazid, Group-E was given isoniazid 50mg/kg/day and rifampicin 100 mg/kg/day, silymarin 200 mg/kg/day and walnut leaf extract 0.2 g/kg orally for 14 days. On day fifteen, terminal blood sampling was done for estimation of serum Aspartate aminotransferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (ALP).
Results: Isoniazid and Rifampicin produced severe hepatotoxicity, depicted by raised mean serum AST (168.20±4.85), ALT (233.60±29.98) and ALP (288.30±19.51). Mean AST (53.30±7.32), ALT (51.80±7.21) and ALP (133.70±7.42) levels were significantly decreased in Group-E, followed by groups C and D.
Conclusion: The combination of silymarin and walnut leaf extract is more effective in preventing liver damage as compared to their separate effects.
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