Comparison of Modified Carbapenem Inactivation Method (mCIM) with Novel Rapid Carbapenemase Detection Method (rCDM) for Detection of Carbapenemase-Producing Organisms from Clinical Isolates

Asad Zafar; Irfan Ali  Mirza; Sakeenah Hussain  Naqvi; Anam  Imtiaz; Adeel  Gardezi; Rabia  Sajjad

doi:10.51253/pafmj.v76i3.11452

Authors

Asad Zafar Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Irfan Ali Mirza Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Sakeenah Hussain Naqvi Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Anam Imtiaz Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Adeel Gardezi Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Rabia Sajjad Department of Microbiology, Armed Forces Institute of Pathology /National University of Medical Sciences (NUMS) Rawalpindi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v76i3.11452

Keywords:

Carbapenemase, Enterobacterales, Rapid Carbapenemase detection method (rCDM).

Abstract

Objective: To determine the diagnostic accuracy of rapid Carbapenemase detection method (rCDM) for the detection of Carbapenemase-producing organisms, keeping the modified carbapenem inactivation method (mCIM) as the reference method

Study Design: Cross-sectional study

Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan, from Jun 21 to May 22.

Methodology: A total of 180 carbapenem-resistant isolates from clinical specimens, including blood samples, respiratory samples (sputum, pleural fluid, endobronchial washings, bronchoalveolar lavage), pus, stool samples, and urine samples of patients of all ages irrespective of gender, were included. All carbapenem-resistant isolates detected using the disc diffusion method were further tested for Carbapenemase production using the novel rapid Carbapenemase detection method (rCDM), and the Clinical Laboratory Standard Institute (CLSI 2021 guidelines) recommended mCIM method.

Results: The specificity, sensitivity, negative predictive value, positive predictive value, and diagnostic accuracy of the rCDM for identifying Carbapenemase-producing organisms were 97.30%, 98.60%, 94.74%, 99.30%, and 99.33%, respectively, using the modified carbapenem inactivation method (mCIM) as the reference method.

Conclusions: The diagnostic accuracy for the detection of Carbapenemase-producing Gram-negative bacteria using rCDM among clinical samples is very high. It is a rapid, reliable, and cost-effective method for the detection and timely reporting of Carbapenemase-producing organisms in healthcare settings.

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