EFFECT OF MONOSODIUM GLUTAMATE ON THE EPITHELIAL HEIGHT OF FALLOPIAN TUBE OF RAT AND ITS PREVENTION WITH
Objective: To study the effect of monosodium glutamate on the fallopian tube of adult Sprague Dawley rat and prevention of this effect with diltiazem.
Study Design: Laboratory based randomized controlled trial.
Place and Duration of the Study: Anatomy Department, Army Medical College, Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad from 9th April – 23rd April 2012.
Material and Methods: In this study 30 adult female Sprague Dawley rats of average weight of 500 g were randomly assigned into three groups A (control group), B and C (experimental groups) with 10 rats in each group. The experimental group B was given 0.08 mg/kg of monosodium glutamate (MSG) orally and experimental group C was given 0.08 mg/kg of MSG and 10 mg/kg of diltiazem in distilled water orally for 14 days. The control group (A) received only laboratory diet prepared at NIH Islamabad with liberal water intake. The rats were sacrificed on the 15th day of the experiment. The fallopian tubes (F.T) were carefully dissected out and fixed in 10% formalin for routine histological examination.
Result: The histological findings in the experimental group B showed evidence of an increase in epithelial height whereas no such finding was observed in groups A and there was a minor increase in epithelial height in group C.
Conclusion: MSG causes an increase in epithelial height of fallopian tubes in adult female rats but treatment with diltiazem prevents this effect.