DAILY VARIATION OF BRAIN PROTEIN IN THE PRE-FRONTAL CORTEX OF ARVICANTHIS ANSORGEI

Abstract

Objective: To examine the daily variation of total protein contents in the pre-frontal cortex of the diurnal rodents, Arvicanthis 
ansorgei.
Study Design: Quasi-experimental study. 
Place and Duration of Study: Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore Pakistan, form 
Jan to May 2023. 
Methodology: Male young rats were forfeited at various zeitgeber times (ZT1, ZT5, ZT9, ZT13, ZT17, and ZT21), ZT0, and ZT12
characterizing lights on and off respectively. The brains were separated on ice and the pre-frontal cortex was isolated. The 
quantity of total protein contents was assessed using the Bradford test on the brain supernatants. 
Results: The total brain protein contents in the prefrontal cortex of Arvicanthis ansorgei follow a circadian variation during 
the 24 hours of the day. Repeated measure ANOVA indicated a significant difference among all the six different time points, 
F (1,5)=595.476, p<0.001.
Conclusion: The first evidence that total protein contents alter at different times of the day in the pre-frontal cortex with a peak
at ZT17 (5h after light off) and low at ZT1 (1h after light on) in diurnal rodents.