SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA

Authors

  • Munnaza Sarfraz Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Amir Rashid Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Midhat Mustafa Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Saleem Ahmad Khan Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Asifa Majeed Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan

Keywords:

ABCA1 gene, DNA sequencing, Dyslipidemia, Low-density lipoproteins, Triglycerides, Type 2 diabetes mellitus

Abstract

Objective: To find the genetic mutation in exon-9 of the ABCA1 gene and relationship of serum low-density lipoprotein levels and serum triglycerides in patients with type 2 diabetes mellitus with and without dyslipidemia.

Study Design: Cross-sectional comparative.

Place and Duration of Study: Department of Biochemistry and Molecular Biology, Army Medical College Rawalpindi, from Jan to Dec 2017.

Methodology: Ninety subjects were selected and divided into three groups, thirty in each. The division was based on newly diagnosed patients of type 2 diabetes with dyslipidemia, newly diagnosed patients of type 2 diabetes and healthy individuals. Genomic DNA was extracted from the blood samples of all subjects. Exon-9 of the ABCA1 gene was amplified through polymerase chain reaction and sequenced on automated DNA sequencer. The biochemical data was analyzed by SPSS-20 and presented in percentage and mean  SD.

Results: The pattern of sequences of ABCA1 gene was found normal in patients of the group I and II through DNA sequencing. The levels of triglycerides were found elevated and mean value was 2.45 ± 0.57 for patients with diabetic dyslipidemia as compared to diabetic and control groups. The levels of low-density lipoproteins were observed as normal with mean value of 1.50 ± 0.22.

Conclusion: The ABCA1 gene did not carry any genetic mutation in exon-9 in studied subjects. The raised level of triglyceride lipoproteins was a typical sign of dyslipidemia with a normal profile of low-density lipoproteins.

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Published

30-10-2020

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Original Articles

How to Cite

1.
Sarfraz M, Rashid A, Mustafa M, Khan SA, Majeed A. SCREENING OF GENETIC MUTATION IN ABCA1 GENE AND RELATIONSHIP OF LOW-DENSITY LIPOPROTEIN AND TRIGLYCERIDES IN PATIENTS WITH TYPE 2 DIABETES WITH AND WITHOUT DYSLIPIDEMIA. Pak Armed Forces Med J [Internet]. 2020 Oct. 30 [cited 2024 Nov. 3];70(5):1271-76. Available from: https://pafmj.org/PAFMJ/article/view/5548