EFFECT OF ALPHA TOCOPHEROL ON CHRONIC STRESS INDUCED DERANGEMENTS INN NEUROPEPTIDEY ON SPRAGUE DAWLEY RATS

  • Saadia Zainab Al-Nafees Medical College & Hospital, Islamabad Pakistan
  • Umar Ali Khan Al-Nafees Medical College & Hospital, Islamabad Pakistan
  • Ghulam Mustafa Lodhi Al-Nafees Medical College & Hospital, Islamabad Pakistan
  • Munazza Asad Al-Nafees Medical College & Hospital, Islamabad Pakistan
Keywords: Alpha-Tocopherol, Neuropeptide Y, Sprague dawley rats

Abstract

Objective: To determine the chronic stress induced decline in plasma Neuropeptide Y (NPY) level and protective effects of Alpha Tocopherol (AT) on maintenance of Neuropeptide Y level.

Study Design: Quasi-experimental study.

Place and Duration of Study: The study settings were Al-Nafees Medical College and animal center of National Institute of Health Islamabad (NIH). Duration of research was one year from May 2015 to May 2016.

Methodology: After taking approval of institutional review board 45 male Sprague Dawley rats with inclusion criteria were chosen and allocated to three groups comprised of n=15. Group I was control, group II restraint stress and group III (restraint stress with supplementation of alpha-tocopherol). After 28 days of restraining stress induction plasma Neuropeptide Y level was determined by sandwich Enzyme Linked Immunosorbent Assay (ELISA).

Results: There was a statistically significant decline (p<0.05) in plasma Neuropeptide Y levels in restraint group as compared to control group and with alpha-tocopherol supplementation group.

Conclusion: Chronic restraint stress induction led to decline in Neuropeptide Y levels and alpha-tocopherol prevented fall in Neuropeptide Y levels.

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Published
2020-06-30
How to Cite
Zainab, S., Khan, U., Lodhi, G., & Asad, M. (2020). EFFECT OF ALPHA TOCOPHEROL ON CHRONIC STRESS INDUCED DERANGEMENTS INN NEUROPEPTIDEY ON SPRAGUE DAWLEY RATS. Pakistan Armed Forces Medical Journal, 70(3), 776-80. Retrieved from https://pafmj.org/index.php/PAFMJ/article/view/4659
Section
Original Articles