EXPRESSION OF GATA 3 IN EPITHELIAL TUMORS

  • Amna Asif Shaukat Khanum Memorial Cancer Hospital, Lahore Pakistan
  • Sajid Mushtaq Shaukat Khanum Memorial Cancer Hospital, Lahore Pakistan
  • Usman Hassan Shaukat Khanum Memorial Cancer Hospital, Lahore Pakistan
  • Noreen Akhtar Shaukat Khanum Memorial Cancer Hospital, Lahore Pakistan
  • Muhammad Azam Shaukat Khanum Memorial Cancer Hospital, Lahore Pakistan
Keywords: Colorectal carcinoma, GATA-3, High grade serous carcinoma, Invasive mammary carcinoma, Prostatic adenocarcinoma

Abstract

Objective: To assess the expression of GATA-3 among epithelial tumors of four organs including invasive mammary carcinoma, prostatic adenocarcinoma, colorectal carcinoma and high grade serous carcinoma of ovary.

Study Design: Case series study.

Place and Duration of Study: The study was performed at Shaukat Khanum Memorial Cancer Hospital and Research Center, from Jul 2017 to Dec 2017.

Methodology: Twenty cases of each of these tumors were collected and stained with ready to use GATA-3 immunohistochemical stain (Cell marque (L50-823) mouse monoclonal antibody) using BOND III Leica auto-mated immunostainer. The expression of the antibody was assessed by two histopathologists. Diffuse nuclear staining in more than 30% of tumor cells was considered as positive.

Results: All the breast carcinomas showed diffuse strong positivity in tumor cells, while all the cases of prostatic adenocarcinoma showed negative staining. Aberrant and focal staining was observed in a few cases of high grade serous and colorectal carcinomas. Three cases of high grade serous carcinoma showed weak nuclear staining in 10% of cells. Only one case of colorectal carcinoma showed weak nuclear blush in 10 to 20% of tumor cells.

Conclusion: GATA-3 was found a sensitive and specific marker for mammary carcinomas. It is a useful diagnostic tool and should be included in a panel of immunohistochemical markers when working on a metastatic tumor of unknown origin.

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Published
2020-01-27
How to Cite
Asif, A., Mushtaq, S., Hassan, U., Akhtar, N., & Azam, M. (2020). EXPRESSION OF GATA 3 IN EPITHELIAL TUMORS. Pakistan Armed Forces Medical Journal, 70(Suppl-1), S20-25. Retrieved from https://pafmj.org/index.php/PAFMJ/article/view/3787
Section
Original Articles