EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE

  • Noreen Anwar Quetta Institute of Medical Sciences, Quetta/National University of Medical Sciences (NUMS) Pakistan
  • Shabnam Hamid Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Amer Nadeem Combined Military Hospital, Quetta/ National University of Medical Sciences (NUMS) Pakistan
  • Ayesha Asad Quetta Institute of Medical Sciences, Quetta National University of Medical Sciences (NUMS) Pakistan
  • Naureen Waseem Islamic Medical College, Sialkot Pakistan
  • Nomana Mehmood Wah Medical College, Wah National University of Medical Sciences (NUMS) Pakistan
Keywords: Fasting blood glucose, Letrozole, Nigella sativa, Polycystic ovarian syndrome, Serum testosterone level

Abstract

Objective: Monitor the ameliorative impact of Nigella sativa on fasting blood glucose and serum testosterone
levels in polycystic ovarian syndrome caused by letrozole in mice.

Study Design: Laboratory based randomized control trial.

Place and Duration of Study: Anatomy department, Army Medical College in teamwork with National Institute
of Health. Time span is Dec 2014 Dec 2015.

Methodology: Total 40 mature female BALB/c mice were chosen and distributed in four groups (ten mice in each one). Group 1 was given normal diet. Rests of three groups were treated with Letrozole at dose of 1 mg/kg body weight once daily for eight weeks. Group 3 was also given Nigella sativa seeds powder at dose of 10 g/kg body weight once daily starting at 22 day and continued up to eight weeks. Group 4 was treated with Nigella sativa oil at a dose of 4 ml/kg body weight once daily starting at 22 day and continued up to eight weeks. At termination of trial, fasting blood glucose level (FBG) was recorded by taking blood sample by glucometer from mouse lateral tail vein after 12 hours fasting. Mice with blood glucose level 250-464 mg/dl were marked hyperglycemic. Final blood sample was taken through cardiac puncture for serum testosterone evaluation.

Results: Fasting blood glucose and Serum testosterone levels were raised in group 2 animals in comparison to
group 1 while they were reduced remarkably in group 3 and 4 in comparison of group 2.

Conclusion: Nigella sativa seeds in both solid and liquid state leave..................

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Published
2021-02-25
How to Cite
Anwar, N., Hamid, S., Nadeem, A., Asad, A., Waseem, N., & Mehmood, N. (2021). EFFECT OF NIGELLA SATIVA ON SERUM TESTOSTERONE LEVELS IN LETROZOLE INDUCED POLYCYSTIC OVARIAN SYNDROME IN MICE. PAFMJ, 71(1), 338-42. https://doi.org/10.51253/pafmj.v71i1.3693
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Original Articles