Determination of Medians of Biochemical Serum Markers in First Trimester of Healthy Pregnant Women in Tertiary Care Setup

  • Shamsa Kanwal Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Naveed Asif Combined Military Hospital Quetta/National University of Medical Sciences (NUMS) Pakistan
  • Shehla Baqai Pak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Aamir Ijaz Rehman Medical Institute, Peshawar Pakistan
  • Kulsoom Bahadur Rehman Medical Institute, Peshawar Pakistan
  • Sumbal Nida Pakistan Air Force Hospital, Islamabad Pakistan
Keywords: Beta Human Chorionic Gonadotropin, Pregnancy-associated plasma protein A, Threatened abortions

Abstract

Objective: To determine medians of pregnancy-associated plasma protein-A (PAPP-A) and free β subunit of Human Chorionic Gonadotropin (hCG-β) in the first trimester of healthy pregnant women visiting tertiary care hospital.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP) from May 2018 to May 2019.

Methodology: Healthy pregnant women with a singleton pregnancy reported for an antenatal checkup in Obstetric OPD, Pak Emirates Military Hospital (PEMH) and Combined Military Hospital (CMH) Rawalpindi were included in the study. PAPP-A level was determined by enzyme-linked immunosorbent assay (ELISA) method using PR 4100 Microplate Reader®, and serum hCG-β were analysed on random access IMMULITE 2000®.

Results: A total of 135 pregnant women were enrolled in the current study. The mean age of subjects was 27.66 ± 4.24 years, mean maternal weight was 59.91 ± 8.87 kgs and mean gestational age was 10.37 ± 1.16 weeks. Median value of PAPP-A was 1289.43 mIU/L (range: 510.8-5965.99) and hCGβ was 120985 mIU/ml (range: 23592-290000).

Conclusion: Median values of PAPP-A and HCGβ can be used to calculate (Multiple of Medians) MoM during the first trimester in tertiary care setup.

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Published
2022-05-01
How to Cite
Kanwal, S., Asif, N., Baqai, S., Ijaz, A., Bahadur, K., & Nida, S. (2022). Determination of Medians of Biochemical Serum Markers in First Trimester of Healthy Pregnant Women in Tertiary Care Setup. Pakistan Armed Forces Medical Journal (PAFMJ), 72(2), 668-71. https://doi.org/10.51253/pafmj.v72i2.2996
Section
Original Articles

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