Determination of Medians of Biochemical Serum Markers in First Trimester of Healthy Pregnant Women in Tertiary Care Setup

Shamsa Kanwal; Naveed Asif; Shehla Baqai; Aamir Ijaz; Kulsoom Bahadur; Sumbal Nida

doi:10.51253/pafmj.v72i2.2996

Authors

Shamsa Kanwal Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Naveed Asif Combined Military Hospital Quetta/National University of Medical Sciences (NUMS) Pakistan
Shehla Baqai Pak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Aamir Ijaz Rehman Medical Institute, Peshawar Pakistan
Kulsoom Bahadur Rehman Medical Institute, Peshawar Pakistan
Sumbal Nida Pakistan Air Force Hospital, Islamabad Pakistan

DOI:

https://doi.org/10.51253/pafmj.v72i2.2996

Keywords:

Beta Human Chorionic Gonadotropin, Pregnancy-associated plasma protein A, Threatened abortions

Abstract

Objective: To determine medians of pregnancy-associated plasma protein-A (PAPP-A) and free β subunit of Human Chorionic Gonadotropin (hCG-β) in the first trimester of healthy pregnant women visiting tertiary care hospital.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP) from May 2018 to May 2019.

Methodology: Healthy pregnant women with a singleton pregnancy reported for an antenatal checkup in Obstetric OPD, Pak Emirates Military Hospital (PEMH) and Combined Military Hospital (CMH) Rawalpindi were included in the study. PAPP-A level was determined by enzyme-linked immunosorbent assay (ELISA) method using PR 4100 Microplate Reader®, and serum hCG-β were analysed on random access IMMULITE 2000®.

Results: A total of 135 pregnant women were enrolled in the current study. The mean age of subjects was 27.66 ± 4.24 years, mean maternal weight was 59.91 ± 8.87 kgs and mean gestational age was 10.37 ± 1.16 weeks. Median value of PAPP-A was 1289.43 mIU/L (range: 510.8-5965.99) and hCGβ was 120985 mIU/ml (range: 23592-290000).

Conclusion: Median values of PAPP-A and HCGβ can be used to calculate (Multiple of Medians) MoM during the first trimester in tertiary care setup.