Establishment of Reference Values of Small Dense Low Density Lipoprotein Cholesterolin Local Population

Iffat Saleem; Muhammad Aamir; Zujaja Hina Haroon; Muhammad Usman Munir; Afshan Yasir; Muhammad Younas

doi:10.51253/pafmj.v75iSUPPL-6.8087

Authors

Iffat Saleem Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan
Muhammad Aamir Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan
Zujaja Hina Haroon, Prof Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan
Muhammad Usman Munir Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan
Afshan Yasir Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan
Muhammad Younas Department of Chemical Pathology & Endocrinology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Pakistan

DOI:

https://doi.org/10.51253/pafmj.v75iSUPPL-6.8087

Keywords:

Coronary Heart Disease, Reference values, Small Dense LDL Cholesterol

Abstract

Objectives: To establish reference interval of small dense low density lipoprotein cholesterol (sdLDL-C) in local population.

Study Design: Cross sectional study.

Place and Duration of Study: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan, from Feb 2021 to Dec 2021.

Methodology: One hundred and twenty healthy reference individuals were enrolled in this study. Blood samples for lipid profile and sdLDL-C were collected after 12-hour fast. SdLDL-C levels were analyzed by automated standardized enzymatic assay on Siemens Advia 1800. The normality of distribution of data was determined by Kolmogorov Smirnov test. Data was analyzed using statistical package for social sciences (SPSS) version 21. Data being non parametric, median and inter quartile range (IQR) were used for expression of quantitative variables. For reference interval of sdLDL-C, 2.5^th and 97.5^th percentiles were used.

Results: A total of 120 healthy participants were enrolled in our study, out of which 84(70%) were male and 36(30%) were female. Median/IQR age of all participants were 43(30 - 51) years, while their median/IQR sdLDL-C level was 0.58(0.48 – 0.73) mmol/L. Reference values of sdLDL-C were 0.33 - 0.99 mmol/L at 2.5^th and 97.5^th percentiles respectively.

Conclusion: Reference interval for sdLDL-C is 0.33 - 0.99 mmol/L in local healthy population. It is recommended to look beyond conventional lipid profile and estimate sdLDL-C which might help in identifying a high risk population in absence of other known risk factors.

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