Hepatitis C Virus RNA and Genotype in Semen of Primitively Diagnosed Patients Infected with HCV-RNA Infection Amongst the Community of Lahore Cantt

Authors

  • Tanveer Hussain Khan Department of Cardiology, Dar-Ul-Shifa Hospital Rawalakot, Azad Jammu Kashmir, Pakistan
  • Muhammad Usman Sheikh Department of Community Medicine, Avicenna Medical & Dental College, Lahore Pakistan
  • Amna Shahid Department of Forensic Medicine, Combined Military Hospital Medical & Dental College, Lahore Pakistan
  • Salman Asghar Medical Officer, Department of General Medicine, Lahore Pakistan
  • Malahat Alina Usman General Physician, Lahore American School, Lahore Pakistan

DOI:

https://doi.org/10.51253/pafmj.v74i5.8069

Keywords:

Genotype, Hepatitis C Virus, Ribonucleic Acid, Seminal Fluid.

Abstract

Objective: To determine whether Hepatitis C virus is present in the seminal fluid of primitively diagnosed patients and subsequent genotyping of semen-positive samples.

Study Design: Cross-sectional study.

Place and Duration of Study: Darul Shifa Clinic and the PCR Lab, Lahore Pakistan, from Apr 2021 to May 2022.

Methodology: We studied 100 patients with diagnosed Hepatitis C infection via Polymerase Chain Reaction. The reverse transcriptase Polymerase Chain Reaction was used to detect the presence of the Hepatitis C virus in the semen of these sample patients.

Results: Mean age of enrolled 100 male patients was 33.86±7.9 years. Only 4% of semen samples tested HCV RNA positive, while 96% were negative. The results of multiplex Polymerase Chain Reaction revealed that 50% of cases showed genotype 2a and 50% cases showed genotype 3a out of 4 positive cases of HCV RNA.

Conclusion:  The observed viral prevalence was relatively low; nevertheless, the potential for pathogen dissemination remains significant. These findings contribute to our understanding of disease transmission dynamics and may have implications for epidemiological modeling and public health interventions.

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Published

31-10-2024

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Original Articles

How to Cite

1.
Khan TH, Sheikh MU, Shahid A, Asghar S, Usman MA. Hepatitis C Virus RNA and Genotype in Semen of Primitively Diagnosed Patients Infected with HCV-RNA Infection Amongst the Community of Lahore Cantt. Pak Armed Forces Med J [Internet]. 2024 Oct. 31 [cited 2024 Dec. 22];74(5):1277-80. Available from: https://pafmj.org/PAFMJ/article/view/8069