Renal Tubular Injury in Albino Rats after using Heavy Metal Copper Dosage:    A Histopathological Study

Hira Kareem; Amna Jahan; Sobia Jahangir; Roop e Zahra; Arslan Haider; Romman ul Haq

doi:10.51253/pafmj.v75iSUPPL-6.8052

Authors

Hira Kareem Department of Pathology, Services Institute of Medical Sciences, Lahore Pakistan
Amna Jahan Department of Pathology, Services Institute of Medical Sciences, Lahore Pakistan
Sobia Jahangir Department of Pathology, University of Health Sciences, Lahore Pakistan
Roop e Zahra Department of Pathology, Services Institute of Medical Sciences, Lahore Pakistan
Arslan Haider Department of Pathology, University of Health Sciences, Lahore Pakistan
Romman ul Haq Department of Radiology, Services Institute of Medical Sciences, Lahore Pakistan

DOI:

https://doi.org/10.51253/pafmj.v75iSUPPL-6.8052

Keywords:

Copper, Diet, Dilatation, Epithelial Cell, Inflammation, Necrosis, Rats, Renal Tubules.

Abstract

Objective: To compare the impact of doses of heavy metals like copper on renal tubules in albino rats.

Study Design: Comparative Cross-sectional study.

Place and Duration of Study: Pathology Department of Services Institute of Medical Science, (Animal House) University of Health Sciences, Lahore Pakistan, from Nov 2018 to May 2019.

Methodology: Two groups of Wistar Albino rodents were categorized as control and study groups consisting of ten rodents. Only standard pellets diet of mice with tap water was given to all control group members. On the contrary, the heavy metal dose was calculated according to the bodyweight of the rodents in the study group. The dose was given to the rodents every alternate day for up to eighteen weeks. At the end of the experiment, changes in renal tubules were compared microscopically and statistically analyzed in both categories, keeping the p-value of <0.05 as significant.

Results: The results showed that morphological changes in renal tubules in tubular epithelial necrosis and dilatation were significantly different in all members of both categories’., epithelial necrosis; (n=10, 100%, p<0.001), followed by dilatation (n=10, 100%, p<0.001), casts (n=10, 100%, p<0.001), basement membrane thickening (n=10, 100%, p<0.001), and vacuolization (n=10, 100%, p<0.001).

Conclusion: This study found that heavy metals like copper can cause renal tubular necrosis, dilatation, and inflammation.

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