Evaluation of Nitric Oxide Activity of Torilis Leptophyllain Indomethacin Induced Gastric Ulcer in Mice


  • Saba batool Department of Pharmacology, Combined Military Hospital, Multan/National University of Medical Sciences (NUMS) Pakistan
  • Saima Noreen Department of Pharmacology, Lahore Medical and Dental College, Lahore Pakistan
  • Tooba Malik Department of Pharmacology, Services Institute of Medical Sciences, Lahore Pakistan
  • Javaria Fatima Department of Pharmacology, Fatima Jinnah Medical University, Lahore Pakistan
  • Bushra Shaheen Department of Pharmacology, Bakhtawar Ameen Medical and Dental College, Multan Pakistan
  • Sheikh Maria Qammar Department of Pharmacology, Rashid Lateef Medical and Dental college, Lahore Pakistan




Gastric ulcer, Indomthacin, Nitric oxide, Torilis leptophylla


Objective: To evaluate the Nitric Oxide activity of Torilis leptophylla in Indomethacin-induced gastric ulcers in mice.

Study Design: Laboratory-based experimental study

Place and Duration of Study: Pharmacology Department, University of Health Sciences, Lahore Pakistan, from Jan to Dec

Methodology: Thirty-six adult healthy male BALB/C mice with a weight range of 25-35 g were equally divided into six
groups and designated as Group-I (Control), Group-II (Positive Control), Groups III to V (Torilis leptophylla extract trial
Groups) and Group-VI (Omeprazole). Gastric ulceration was induced by a single dose of Indomethacin (20mg/kg) in Groups
II–VI. The plant extract was administered in Groups III-V (100, 200, 300 mg/kg) whereas Omeprazole (3mg/kg) in Group-VI
by gavage daily for three days as treatment of gastric ulceration. Nitric Oxide content was measured in gastric juice and serum of all groups by Nitric Oxide assay kit.

Results: The positive Control-Group showed little Nitric Oxide in gastric juice and serum. Torilis leptophylla treated Groups
(100 mg/kg, 200mg/kg and 300 mg/kg) showed significantly raised Nitric Oxide content in both gastric juice and serum than the Control Group (p<0.001).

Conclusion: The study has determined that Torilis leptophylla possesses antiulcer and mucosal protective effects owing to the antioxidant activity of nitric oxide. This supports the use of the plant for the treatment of gastric ulcers.


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How to Cite

batool, S., Noreen, S., Malik, T., Fatima, J., Shaheen, B., & Qammar, S. M. (2023). Evaluation of Nitric Oxide Activity of Torilis Leptophyllain Indomethacin Induced Gastric Ulcer in Mice. Pakistan Armed Forces Medical Journal, 73(6), 1590–1593. https://doi.org/10.51253/pafmj.v73i6.6904



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