Anti-Inflammatory Activity of Crude Extract of Aesculus Indica in Comparison with Aspirin on Carrageenan Induced Paw Edema in Rats

Abstract

Objective: To determine the anti-inflammatory activity of crude extract of Aesculus Indica in rat model.Study design: Comparative studyPlace and duration of study: Pharmacy Department, Bahauddin Zakria University, Multan, Pakistan, from May 2017 to October 2017.Methodology:Twenty four healthy male and female Wistar rats (200-220gm) were included in the study, each rat was given single injection of 0.1 ml of 1% carrageenan, in normal saline in sub-planter region of the right paw for induction of edema. The rats were randomly divided by lottery method in to four groups; Group-1 served as positive control; Group 2 and 3 received crude extract of Aesculus Indica 100 mg/kg and 300mg/kg respectively; Group 4 was given aspirin at the dose of 12 mg/kg. The measurement of paw volume was made immediately by displacement technique using the plathysmometer before and at 1st, 2nd, 3rd and 4th hour after the carrageenan injection.Results: The result of the study confirmed the anti-inflammatory action of crude extract of Aesculus indica both at 100 mg/kg and 300 mg/kg. When group 1 was compared with group 2, 3 and 4, it revealed significant difference with p-value 0.002, 0.042 and 0.002 respectively. Similarly, comparison of group 2 with group 3 showed significant difference with p-value 0.02 while insignificant difference was found between group 3 and 4. Significant difference of p-value 0.03 was found when group 2 and 4 were compared.Conclusion: Crude extract of the Aesculus Indica showed significant anti-inflammatory properties which at high doses were almost comparable with that of aspirin.