ARSENIC INDUCED HISTOMORPHOLOGICAL ALGTERATIONS IN SIZE OF HEPATIC LOBULE AND AMELIORATIVE EFFECTS OF LAGENARIA SICERARIA

Abstract

Objective: To study the effects of Lagenaria siceraria and arsenic on size of Hepatic lobule of Sprague Dawley rat. Study Design: Laboratory-based randomized controlled trial. Place and Duration of Study: Army Medical College, Rawalpindi, and National Institute of Health (NIH), Islamabad, for eight weeks, from 20th Mar 2017 to 14th May 2017.
Material and Methods: Fifty Sprague Dawley rats both male and female selected randomly and broadly allocated into five groups, each with 10 animals. Groups A and B animals were controls, C, D and E served as experimental groups. Sodium arsenite 5mg/kg body weight was initially given for a period of 4 weeks to the experimental groups C, D and E. After 4 weeks of therapy, the animals of groups A and C were sacrificed for histopathological study of liver to see the immediate effects of arsenic. Within the next 4 weeks group D animals were retained without any intervension. Group E animals were administered sodium arsenite (5mg/kg body weight) along with Lagenaria siceraria at a dose of 100mg/kg bodyweight. Group B served as a control for experimental group D and E. Animals of group B, D and E were sacrificed after last dose at the end these 4 weeks. Liver was removed and prepared for histological study. Size of hepatic lobule was studied. Data were analysed by SPSS V 22. A p-value 0.05 was considered statistically significant. Results: Moderate increase in the size of hepatic lobule was seen in group C as compared to control group A. Group.....