Beetroot (Beta vulgaris) Extract Effectiveness as an Anti-Aging, Nephroprotective, and Anti-Hypercholesterolemic Agent in D-Galactose Induced Wistar Rats

Jesslyn; Linda Chiuman; Chrismis Novalinda  Ginting; Sulaiman Delrizal; Sahna Ferdinand

doi:10.51253/pafmj.v75i5.12888

Authors

Jesslyn Department of Biomedical Science, Universitas Prima, Indonesia https://orcid.org/0009-0001-5108-8562
Linda Chiuman Department of Biomedical Science, Universitas Prima, Indonesia
Chrismis Novalinda Ginting Department of Biomedical Science, Universitas Prima, Indonesia
Sulaiman Delrizal Department of Biomedical Science, Universitas Prima, Indonesia
Sahna Ferdinand Department of Biomedical Science, Universitas Prima, Indonesia

DOI:

https://doi.org/10.51253/pafmj.v75i5.12888

Keywords:

Beetroot Extract, Organoprotector, Skin Aging

Abstract

Objective: to determine the effectiveness of beet root extract as an anti-aging, nephroprotector, and anti-hypercholesterolemia in Wistar rats induced with D-galactose to maximize the use of beetroot as a health supplement.

Study Design: Laboratory-based experimental study.

Place and Duration of Study: Laboratory of Faculty of Medicine, Universitas Prima, Indonesia, and Pharmacy Laboratory, Universitas Sumatera Utara, Indonesia, from Jun 2023 to Aug 2023.

Methodology: The study sample consisted of 25 male Wistar rats aged six to eight weeks that were obtained from the Pharmacy Laboratory and had gone through the inclusion and exclusion criteria. The sample was then induced by D-galactose and given an intervention using beetroot extract and vitamin C.

Results: Beetroot extract at a dose of 500 mg/kg body weight showed more significant results as anti-aging, nephroprotector, and anti-hypercholesterolemic compared to 250 mg/kg body weight. Mean value of Group C for anti-aging was 136.26±15.16 (keratinocyte cells) and 35.93±10.64 (fibroblast cells), which was slightly lower than Group D, 136.86±4.29 (keratinocyte cells) and 43.80±5.80 (fibroblast cells). Ureum mean value of Group C for nephroprotector was 79.00±3.16, which was significantly lower than Group D (127.20±6.26) and Group E (105.00±4.63), but creatinine in Group E was the lowest with a mean value of 105.00±4.63 compared to Group C (0.83±0.08) and Group D (0.74±0.07). Group E had the lowest mean value, 105.00±4.63, for total cholesterol compared to Group C (165.00±16.12) and Group D (127.20±6.26).

Conclusion: This experimental study proved the positive effects of beet root extract as anti-aging, nephroprotective, and antihypercholesterolemic on the

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