EVALUATION OF MINIMAL INHIBITORY CONCENTRATIONS OF CEFTAROLINE, TEICOPLANIN AND DAPTOMYCIN FOR TREATMENT OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN A TERTIARY CARE SETTING

Aneela Khawaja; Muhammad Abid Farooque; Faiqa Arshad; Namra Yunus; Nusrat Alavi; Qurat-ul Ain; Aiman Naveed

doi:10.51253/pafmj.v73i6.12718

Authors

Aneela Khawaja Department of Pathology, Combined Military Hospital/ National University of Medical Sciences (NUMS), Lahore Pakistan
Muhammad Abid Farooque Department of Pathology, Combined Military Hospital/ National University of Medical Sciences (NUMS), Lahore Pakistan
Faiqa Arshad Department of Pathology, Gujranwala Medical College, Gujranwala Pakistan
Namra Yunus Department of Pathology, Fatima Memorial College of Medicine and Dentistry, Lahore Pakistan
Nusrat Alavi Department of Pathology, RMDC/PRTH, Lahore Pakistan
Qurat-ul Ain Department of Pathology, Combined Military Hospital/ National University of Medical Sciences (NUMS), Lahore Pakistan
Aiman Naveed Department of Pathology, Combined Military Hospital/ National University of Medical Sciences (NUMS), Lahore Pakistan

DOI:

https://doi.org/10.51253/pafmj.v73i6.12718

Keywords:

Ceftaroline, Daptomycin, Methicillin resistant Staphylococcus aureus (MRSA), Minimal Inhibitory Concentration (MIC), Teicoplanin.

Abstract

Objective: To evaluate Minimal Inhibitory Concentrations of ceftaroline, teicoplanin & daptomycin for treatment of methicillin
resistant Staphylococcus aureus in a tertiary care setting.
Study Design: Experimental study.
Place and Duration of Study: Department of Pathology, Microbiology Laboratory from Nov 2021 to Oct 2022.
Methodology: All the isolated Staph. aureus was processed and identified by colony morphology on blood agar, gram stain,
and biochemical tests i.e., catalase, coagulase and DNAase test. Minimal Inhibitory Concentration was evaluated using Estrips for ceftaroline, teicoplanin & daptomycin for all the MRSA strains during the study period.
Results: A total of 924 S.aureus strains were processed, and 270 (29.22%) MRSA were recovered during the study period. The
highest percentage 50 was observed in sputum (n=2), followed by 41.66% in endobronchial washing (n=10), pus 160 (31.25%),
tissue 30 (31.25%), pus swab 50 (29.76%), high vaginal swab 2 (25%), and least in blood 16 (14.54%). MICs for all the MRSA
isolates to teicoplanin were in susceptible range (≤8µg/ml). MIC of 22 (8.14%) and 14 (5.18%) MRSA isolates for Ceftaroline
and daptomycin were in susceptible dose-dependent (SDD=2-4µg/ml) range.
Conclusion: The diagnostic modality, antimicrobial susceptibility testing, and MIC determination were found to be the best
approach for adequate management of MRSA.