Comparison of Interferon-Inducible Patient-10 and Chemokine Ligand-2 Expression in COVID-19 Patients

Nayab Khalid; Mehwish Qamar; Sadia Syed; Ibrahim Liaqat; Muhammad Irfan ul Akbar Yousufzai; Summyah Niazi

doi:10.51253/pafmj.v74i1.11796

Authors

Nayab Khalid Department of Physiology, Islam Medical and Dental College, Sialkot, Pakistan
Mehwish Qamar Department of Physiology, Islam Medical and Dental College, Sialkot, Pakistan
Sadia Syed Department of Forensic Medicine & Toxicology, Shifa College of Medicine, Islamabad, Pakistan
Ibrahim Liaqat Department of Physiology, Islam Medical and Dental College, Sialkot, Pakistan
Muhammad Irfan ul Akbar Yousufzai Department of Physiology, Sahara Medical College, Narowal Pakista
Summyah Niazi Department of Physiology, Quetta Institute of Medical Sciences, Quetta Pakistan

DOI:

https://doi.org/10.51253/pafmj.v74i1.11796

Keywords:

COVID-19, ELISA test, Inflammatory cytokines

Abstract

Objective: To compare interferon-inducible patient-10 and chemokine lisgand-2 expression in COVID-19 Patients.

Study Design: Cross-sectional study.

Place and Duration of Study: University of Lahore Teaching Hospital, Lahore Pakistan, from Dec 2020 to Mar 2021.

Methodology: We assessed the level of cytokines in forty-five COVID-19 patients and compared it with the 45 healthy
individuals. The pro-inflammatory cytokines IP-10 and CCL-2 levels were evaluated in patients and healthy people via ELISA
kits.

Results: The study included ninety (n=90) patients. Our data suggested that the serum levels of IP-10 and CCL-2 were
increased (8.5±1.5 ng/ml and 11.89±4.55 pg/ml) significantly in patients with COVID-19 as compared to healthy Controls
(4±0.5ng/ml and 6.15±1.91 pg/ml). A significant increase was recorded in INR, PT (sec) and APTT (sec) levels in COVID-19
patients. Whereas the platelets count (109/ml) was decreased significantly in COVID-19 patients (220±42) as compared to
healthy individuals (419±69). Significant elevation of D-Dimer (ng/ml) was recorded in patients with COVID-19.

Conclusion: Our data suggested a significantly increased level of IP-10 and CCL-2 in COVID-19-associated patients, which
may be of essential diagnostic and prognostic significance for these biomarkers.