Does Vitamin E Protect the Male Reproductive System Against the Noise-Induced Stress?


  • Maria Yousaf Department of Anatomy, Islamic International Medical College, Rawalpindi Pakistan
  • Humaira Ali Department of Anatomy, Swat Medical College, Swat Pakistan
  • Liaqat Ali Department of Anatomy, Swat Medical College, Swat Pakistan
  • Nomana Mahmood Department of Anatomy, Wah medical college, Wah Pakistan
  • Shazia Imran Department of Anatomy, National University of Sciences & Technology (NUST) School of Health Sciences, Islamabad Pakistan
  • Kishwar Naheed Department of Anatomy, Watim Medical and Dental College, Islamabad Pakistan
  • Amna Shoaib Al Nafees Medical College, Islamabad Pakistan



Alph-tocopherol, Antioxidant, Infertility, Noise Stress, Testosterone


Objective: To investigate how Tocopherols shield testis' histomorphology in adult rats subjected to noise stress.
Study Design: Laboratory based-experimental study.
Place and Duration of Study: National Institute of Health, Islamabad Pakistan, from Sep 2022 to Sep 2023.
Methodology: A total of 90 albino rats, with weights ranging from 250 to 300 grams, were included in the study. Three Groups
were created: Group-A, Control, and Group-B, daily noise exposure (100 dB); Group-C was administered with Alpha Tocopherol 40 IU/kg dissolved in 0.5 ml corn oil and subjected to noise (100 dB) daily. The seminiferous tubules' germinal
epithelium's diameter and height were measured using Hematoxylin and Eosin and Periodic Acid Schiff staining.
Results: The study compared gross, microscopic, and biochemical parameters between the Experimental and Control Groups.
Group A exhibited an average testicular volume of 1.82±0.05 gms, a mean tubular diameter of 253.72±4.46 µm, and a germinal
epithelial height of 56.47±2.31 µm. Group-B's testicular volume was 1.13±0.07 gms, accompanied by a decrease in tubular
diameter to 182.15±5.05 µm and epithelial height to 36.80±2.79 µm and in comparison to Group-B, Group-C exhibited
enhancements in terms of tubular diameter (251.86±2.00 µm, p<0.001) and epithelial height (55.63±2.02 µm, p<0.001), resulting
in a reduced testicular volume (1.181±0.03 gms, p<0.001).
Conclusion: Noise-induced histomorphological changes in the testis lowered sperm maturation in the germinal cells and
significantly reduced testicular volume. Moreover, α-Tocopherol has a protective effect on histomorphological changes caused
by noise stress.


Download data is not yet available.


Metrics Loading ...


Billman GE. Homeostasis: The Underappreciated and Far Too

Often Ignored Central Organizing Principle of Physiology. Front

Physiol 2020; 11: 200.

Daiber A, Kröller-Schön S, Oelze M, Hahad O, Li H, Schulz R, et

al. Oxidative stress and inflammation contribute to traffic noiseinduced vascular and cerebral dysfunction via uncoupling of

nitric oxide synthases. Redox Biol 2020; 34: 101506.

Masruri B, Alimohammadi I, Abyaz MR, Moghadasi N,

Yekzamani P, Mohammadzadeh Asl B, et al. Concurrent Lead

and Noise Exposure Effects on Testicular Tissue of Rat: An

Experimental Study. J Family Reprod Health 2022; 16(2): 132-

Arminjon M. Birth of the allostatic model: From Cannon’s

biocracy to critical physiology. Journal of the History of Biology

; 49(2): 397-423.

Krzastek SC, Farhi J, Gray M, Smith RP. Impact of

environmental toxin exposure on male fertility potential. Transl

Androl Urol 2020; 9(6): 2797-2813.

Wang M, Wang Q, Du Y, Jiang H, Zhang X. Vitamins combined

with traditional Chinese medicine for male infertility: A

systematic review and meta-analysis. Andrology 2020; 8(5):


Sabetian S, Jahromi BN, Vakili S, Forouhari S, Alipour S. The

Effect of Oral Vitamin E on Semen Parameters and IVF

Outcome: A Double-Blinded Randomized Placebo-Controlled

Clinical Trial. Biomed Res Int 2021; 2021: 5588275.

Meli R, Monnolo A, Annunziata C, Pirozzi C, Ferrante MC.

Oxidative Stress and BPA Toxicity: An Antioxidant Approach

for Male and Female Reproductive Dysfunction. Antioxidants

; 9(5): 405.

Ojo OA, Nwafor-Ezeh PI, Rotimi DE, Iyobhebhe M, Ogunlakin

AD, Ojo AB, et al. Apoptosis, inflammation, and oxidative stress

in infertility: A mini review. Toxicol Rep. 2023; 10: 448-462.

Checa J, Aran JM. Reactive Oxygen Species: Drivers of

Physiological and Pathological Processes. J Inflamm Res 2020;

: 1057-1073.

Uwejigho RE, Iteire KA, Enemali FU. Psychosocial Stress and

Fertility: The Preventive Potentials of Vitamin E. J Anat Soc

India 2023; 72(3): 229-38.

Hidayatik N, Purnomo A, Fikri F, Purnama MTE. Amelioration

on oxidative stress, testosterone, and cortisol levels after

administration of Vitamins C and E in albino rats with chronic

variable stress. Vet World 2021; 14(1): 137-143.

Farahavar A, Rostami Z, Alipour D, Ahmadi A. The effect of

pre-breeding vitamin E and selenium injection on reproductive

performance, antioxidant status, and progesterone concentration

in estrus-synchronized Mehraban ewes. Trop Anim Health Prod

; 52(4): 1779-1786.

Alsemeh AE, Ahmed MM, Fawzy A, Samy W, Tharwat M, Rezq

S, et al. Vitamin E rescues valproic acid-induced testicular injury

in rats: Role of autophagy. Life Sci 2022; 296: 120434.

Sies H. Oxidative Stress: Concept and Some Practical Aspects.

Antioxidants 2020; 9(9): 852.

Chen H, Wang Y, Luo J, Kang M, Hou J, Tang R, et al.

Autophagy and apoptosis mediated nano-copper-induced

testicular damage. Ecotoxicol Environ Saf 2022; 229: 113039.

Rahman A, Mirbahari SN, Ahmadi R, Gohari A, Eshghjoo S.

Effects of mobile phone radiation and noise pollution on serum

levels of LDH, thyroid hormones, liver enzymes, cortisol, and

reproductive system in male Wistar rats. J Biol Stud 2022; 5(1

(Special issue)): 72-84.

Abouee-Mehrizi A, Saed-Moucheshi S, Rasoulzadeh Y,

Mehdipour A, Mohammadoghli-Reyhan F, Iraji Z, et al.

Toxicological Effects of Simultaneous Exposure to Toluene and

Noise on some Sexual and Stress Parameters in New Zealand

White Rabbits. Pollution 2023; 9(1): 126-138.

Thanh TN, Van PD, Cong TD, Le Minh T, Vu QH. Assessment of

testis histopathological changes and spermatogenesis in male

mice exposed to chronic scrotal heat stress. J Animal

BehavBiometeorol 2020; 8 (3): 174-180.

Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The

Chemistry of Reactive Oxygen Species (ROS) Revisited:

Outlining Their Role in Biological Macromolecules (DNA,

Lipids and Proteins) and Induced Pathologies. Int J Mol Sci 2021;

(9): 4642.




How to Cite

Yousaf M, Humaira Ali, Liaqat Ali, Nomana Mahmood, Shazia Imran, Kishwar Naheed, et al. Does Vitamin E Protect the Male Reproductive System Against the Noise-Induced Stress?. Pak Armed Forces Med J [Internet]. 2024 Apr. 29 [cited 2024 May 24];74(2):512-6. Available from:



Original Articles

Most read articles by the same author(s)