Comparative Analysis of Human Cystatin C Levels in Migraine without Aura and Healthy Controls

Mariyam Khan; Maham Tahir; Sunia Hameed; Muhammad Umar Ali Khan; Mehwish Qamar; Mamoona Shafiq

doi:10.51253/pafmj.v73i4.10867

Authors

Mariyam Khan Department of Physiology, Nishtar Medical University, Multan Pakistan
Maham Tahir Department of Physiology, Islam Medical and Dental College, Sialkot Pakistan
Sunia Hameed Department of Radiology, Mukhtiar A Sheikh Hospital, Multan Pakistan
Muhammad Umar Ali Khan Department of Histopathology, Bakhtawar Amin Hospital, Multan Pakistan
Mehwish Qamar Department of Physiology, Islam Medical and Dental College, Sialkot Pakistan
Mamoona Shafiq Department of Physiology, Islam Medical and Dental College, Sialkot Pakistan

DOI:

https://doi.org/10.51253/pafmj.v73i4.10867

Keywords:

Cystatin C, Migraine, Protective role, Oxidative stress

Abstract

Objective: To compare the levels of human cystatin c (novel biomarker) as well as the association of other parameters in
migraine patients without aura and healthy controls.

Study Design: Cross-sectional, comparative study.

Place and Duration of Study: The research was conducted at IMBB Physiology Department, University of Lahore, Lahore
Pakistan, from May 2021 to Jun 2022.

Methodology: After taking history, a detailed clinical examination was conducted of participants in both cases and controls of
the study. A proforma was filled and a non-probability convenience sampling technique was used. The blood samples were
drawn, centrifuged to isolate serum and analyzed concentration of serum cystatin C levels were measured by using enzyme
linked immunosorbent assay (ELISA). SPSS version 22 was used for statistical analysis. Mann-Whitney U test was used to
determine the median IQR values of both groups and p-value<0.05 was considered significant.

Results: Among cases 19(47.5%) were male and 21(52.5%) were female. Mean age of cases and controls was 25.57±5.93 and
26.15±7.19 years. The most frequent occupation among cases and controls was student, doctors, nurses and house wives.
Among cases only 3(7.5%) patients were currently smokers while among controls 3(7.5%) were currently smokers. Among
cases body mass index of 11(27.5%) patients were 30 and 4(10%) participants in controls had body mass index as 30. The most
frequent symptom was photophobia followed by nausea, phonophobia, vomiting and atypical features. All these features
were significantly higher

Conclusion: Study concluded that patients with migraine without aura have elevated levels of cystatin C in their