Association of Cyclooxygenase 1 (COX1) Polymorphism at SNP RS1330344 with Aspirin Resistance in Pakistani Patients of Ischemic Heart Disease

Mudassar Noor; Syed Zubair Hussain Shah; Usman Nawaz; Qaiser Mansoor

doi:10.51253/pafmj.v74i2.10628

Authors

Mudassar Noor Department of Pharmacology, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Syed Zubair Hussain Shah Department of Biochemistry, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Usman Nawaz Department of Pharmacology, Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Qaiser Mansoor Department Biomedical Engineering, Institute of Biomedical & Genetic Engineering

DOI:

https://doi.org/10.51253/pafmj.v74i2.10628

Keywords:

Aspirin Resistance, Cyclooxygenase 1 Polymorphism, Ischemic Heart Disease, Platelet aggregation, Restriction Fragment Length (RFLP), Single Nucleotide (SNP) rs1330344

Abstract

Objective: To find an association between a single nucleotide polymorphism (SNP) rs1330344 in cyclooxygenase-1 gene with Aspirin resistance in patients with cardiovascular disease in the Pakistani population.

Study Design: Cross-sectional

study Place and Duration: Pharmacology Department, National University of Medical Sciences, Pakistan, in collaboration with the National Institute of Heart Diseases and Institute of Biomedical and Genetic Engineering Islamabad, Pakistan, from Oct 2018 to Dec 2021.

Methodology: The Study was conducted on 384 patients (272 males and 112 females) with ischemic heart disease. Patients on Aspirin for at least seven days were selected. Platelet aggregation was performed using a Light Transmission Aggregatometer and arachidonic acid as an agonist. DNA extraction was done using the kit method (Invitrogen, Thermofisher). Then, Polymerase Chain Reaction, Restriction Fragment Length Polymorphism, and gel electrophoresis were used to identify SNP rs1330344.

Results: In this study, 14.0% (n=54) and 86.0% (n=330) of ischemic heart disease patients were resistant to Aspirin and Aspirin, respectively. The genotyping of Cyclooxygenase 1, SNP rs1330344 showed 24.41% (n=94),17.56% (n=67), and 57.98% (n=223) patients had wild type allele (CC), homozygous (TT) and heterozygous (CT) genotype respectively. Gel electrophoresis results for allelic identification were correlated with the anti-platelet efficacy of Aspirin among ischemic heart disease patients. No association was found between a polymorphic form in SNP and the development of aspirin resistance.

Conclusion: Aspirin resistance is not associated with any specific polymorphic form of Cyclooxygenase-1 at SNP rs1330344 in the Pakistani population.

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