Evaluation of Penicillin Binding Protein 2a (PBP-2a) Latex Agglutination Assay for Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Blood Culture Growth

Riffat Bushra; Sakeenah Hussain Naqvi; Irfan Ali Mirza; Anam Imtiaz; Rabia Sajjad; Maria Mushtaq Gill

doi:10.51253/pafmj.v74i6.10498

Authors

Riffat Bushra Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Sakeenah Hussain Naqvi Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Irfan Ali Mirza Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Anam Imtiaz Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Rabia Sajjad Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Maria Mushtaq Gill Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v74i6.10498

Keywords:

Cefoxitin, Latex agglutination, mecA gene, Methicillin-Resistant Staphylococcus aureus (MRSA), Penicillin Binding Protein 2a (PBP2a).

Abstract

Objective: To determine in vitro activity of Penicillin Binding Protein 2a Latex Agglutination Assay for Rapid Identification of Methicillin-Resistant Staphylococcus aureus isolated from positive blood culture growths.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, Jan to Jun 2022.

Methodology: Staphylococcus aureus isolates were taken from a subculture of blood culture samples flagged positive by the BactAlert automated blood culture system. A total of 107 isolates were included in the study. A latex agglutination assay was used to identify Methicillin-Resistant Staphylococcus aureus and non-Methicillin-Resistant Staphylococcus aureus isolates. The Cefoxitin disc diffusion test also tested the isolates, keeping it the reference method.

Results: Out of 107 samples, 92 isolates were identified as Methicillin-Resistant Staphylococcus aureus by latex agglutination and Cefoxitin disc diffusion. The rest of the isolates were identified as Methicillin-Susceptible Staphylococcus aureus.

Conclusion: The latex agglutination assay is a highly sensitive, cost-effective, less time-consuming, and accurate test that may be used in routine laboratories for rapid identification of staphylococcus aureus isolates as Methicillin-Resistant Staphylococcus aureus.

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