Pooling of Urine Specimens for Diagnosis of Asymptomatic Chlamydia Trachomatis Infection by PCR in a Population of Low Frequency, a Cost-Saving Technique for Epidemiological and Screening Programs

Nawwal Naeem Chaudhary; Irfan Ali  Mirza; Umar  Khurshid; Ammarah Iqbal  Muhammad; Shazia  Gullalai; Sakeenah Hussain  Naqvi

doi:10.51253/pafmj.v74iSUPPL-2.10387

Authors

Nawwal Naeem Chaudhary Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Irfan Ali Mirza Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Umar Khurshid Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Ammarah Iqbal Muhammad Department of Gynecology & Obstetrics, Combined Military Hospital/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Shazia Gullalai Department of Gynecology & Obstetrics, Combined Military Hospital/National University of Medical Sciences (NUMS), Rawalpindi Pakistan
Sakeenah Hussain Naqvi Department of Microbiology, Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS), Rawalpindi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v74iSUPPL-2.10387

Keywords:

Chlamydia Trachomatis, Pooling, PCR, Urine.

Abstract

Objective: To describe the frequency of Chlamydia trachomatis in local community visiting a tertiary care hospital and to estimate the cost saving achieved as a result of pooling strategy.

Study Design: Cross-sectional study.

Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from Jan 2022 to Jun 2022.

Methodology: A pool of three urine samples was created after individual manual DNA extraction of each sample and tested by RT PCR. Any pool signaling positive was identified and all samples in that pool were retested individually to determine the positive sample. A total of 66 asymptomatic young people including males and females were tested.

Results: The frequency of Chlamydia trachomatis was found to be 7.57%. About 22 pools were created resulting in a 48.0% savings in costs.

Conclusion: The Pooling strategy adopted with the objective of saving test costs resulted in getting timely and reliable results in a resource limited setting. It also provided with the means to keep Sexually Transmitted Infections detection programs going on at various healthcare levels and in screening a larger number of populations.

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