Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method

Authors

  • Athar Iqbal Paracha Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Zujaja Hina Haroon Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Muhammad Aamir Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Saima Bashir Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Afshan Bibi Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Haroon Javaid HI-TECH Institute of Medical Sciences, Taxila/National University of Medical Sciences (NUMS) Pakistan

DOI:

https://doi.org/10.51253/pafmj.v73i1.6744

Keywords:

Hypoalbuminemia, Ion selective electrode (ISE), Serum sodium measurement

Abstract

Objective: To evaluate the effect of low serum Albumin levels on serum sodium measurement when analyzed by the indirect Ion Selective Electrode (ISE) method and to compare the results with the direct Ion selective electrode (ISE) method.

Study Design: Cross-sectional study

Place and Duration of Study: Department of Chemical Pathology, Armed Forces Institute of Pathology, Rawalpindi Pakistan, from Jan to Mar 2021.

Methodology: Patients of either gender, aged 18 to 70 years, who were admitted to the Intensive Care Unit of Combined Military Hospital, Rawalpindi, were selected. A total of 200 blood samples were collected in a gel tube. Serum samples were analyzed for albumin and sodium within two hours of sample collection. Sodium levels were measured concurrently by both direct and indirect ISE methods. The difference in results between these two techniques was studied.

Results: Hypoalbuminemia was detected in 176(88%) patients, while 24(12%) had normal albumin levels. In
Hypoalbuminemic patients, serum sodium measurements were higher using the indirect ISE method(134.07±5.55) compared to the direct ISE method (130.95±6.04); the difference between the two techniques was statistically significant (p-value <0.001).Pearson correlation coefficient (r-value = -0.86, p-value <0.001) revealed a symmetrical increase in differences between the two methods as the albumin level decreased.

Conclusion: In Hypoalbuminemic patients, the indirect ISE method gave falsely raised results of serum sodium. In such patients, serum sodium measurement by the Direct ISE method offers more accurate and consistent electrolyte results.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

24-02-2023

How to Cite

1.
Paracha AI, Haroon ZH, Aamir M, Bashir S, Bibi A, Javaid H. Comparing the effect of Hypoalbuminemia on Sodium measured by Indirect versus Direct Ion Selective Electrode Method. Pak Armed Forces Med J [Internet]. 2023 Feb. 24 [cited 2024 May 25];73(1):155-58. Available from: https://pafmj.org/PAFMJ/article/view/6744

Issue

Section

Original Articles

Most read articles by the same author(s)

1 2 3 4 5 > >>