Antiviral Therapeutic Monitoring: Role of Hepatitis C Virus Core Antigen versus Hepatitis C Virus RNA by Polymerase Chain Reaction

  • Hajra Farooq Chughtai Lab, Lahore Pakistan
  • Muhammad Zaheer Iqbal Chughtai Lab, Lahore Pakistan
  • Waheed U Zamaan Tariq Chughtai Lab, Lahore Pakistan
  • Omar Rasheed Chughtai Chughtai Lab, Lahore Pakistan
  • Asad Ali Choudhry Al Raee Hospital, Gujranwala Pakistan
  • Saadiya Mushtaq Combined Military Hospital Lahore/ National University of Medical Science (NUMS)Pakistan
Keywords: Hepatitis C virus, Hepatitis C virus core antigen, Polymerase chain reaction

Abstract

Objective: To study the association between hepatitis C virus viral load by real-time PCR and core antigen value of HCV and define an antiviral treatment monitoring cut-off value for HCV core antigen.

Study Design: Cross-sectional validation study.

Place and Duration of Study: Department of Infectious Diseases, Chughtai Lab Lahore Pakistan, from Jun 2017 to Mar 2018. Methodology: To establish the association between these two parameters, we took a hundred positive plasma samples for HCV RNA and subjected them to an HCV Core antigen test. Furthermore, the samples were divided into three categories based on their viral load; <2000 IU/ml, 2000-10,000 IU/ml and >10000 IU/ml.

Results: Our results showed that the hepatitis C virus core antigen was concordant with hepatitis C virus RNA by PCR when the viral load was above 2000 IU/ml. Below the HCV RNA load of 2000 IU/ml, the HCV core antigen had a sensitivity of 94.95% and specificity of 88.89%.

Conclusion: In patients having a viral load above 2,000 IU/ml, the hepatitis C virus core antigen value can be used as a marker for diagnosis and monitoring antiviral therapy. After the antiviral treatment, HCV RNA real-time PCR should be performed to validate viral clearance.

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Published
2022-12-29
How to Cite
Farooq, H., Iqbal, M. Z., Zamaan Tariq, W. U., Chughtai, O. R., Choudhry, A. A., & Mushtaq, S. (2022). Antiviral Therapeutic Monitoring: Role of Hepatitis C Virus Core Antigen versus Hepatitis C Virus RNA by Polymerase Chain Reaction. Pakistan Armed Forces Medical Journal, 72(6), 1933-36. https://doi.org/10.51253/pafmj.v72i6.5521
Section
Original Articles