EFFECT OF HORMON REPLACEMENT THERAPY ON TOTAL BODY WEIGHT, GROSS APPEARANCE, RELATIVE ORGAN EIGHT AND VOLUME OF LIVER IN OVARIECTOMIZED RAT MODEL
Objective: To evaluate the effects of hormone replacement on gross appearance, total body weight and volume of liver in ovariectomized rats.
Study Design: Laboratory-based experimental study.
Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi, in collaboration with National Institute of Health, Islamabad, from Mar to Jun 2019.
Methodology: Forty, female, healthy, non-pregnant, Sprague dawley rats, 5-6 months of age, weighing 250 ± 10g, were randomly divided into 4 equal groups (A, B, C and D). Group A served as control and group B was sham operated. Group C i.e. ovariectomized group underwent ovariectomy but was kept untreated. Group D under-went ovariectomy and was treated with injection estradiol (Inj Gravibinan, 2ml, Medipharm. Mfg. Co, Ltd) 2.5 mg/kg via subcutaneous route once weekly for 60 days. All animals were sacrificed after the last injection of estradiol as hormone replacement therapy (HRT) to group D. Gross parameters of liver were observed. Results were analyzed using SPSS version 22. The p-value of ≤0.05 was considered as significant.
Results: Ovariectomized (OVX) group showed marked change in colour and consistency of liver, increased total body weight and volume of liver. While these changes started reverting back towards normality in treated group.
Conclusions: Injection of hormone replacement therapy improved the gross parameters of liver in treated group, 12 weeks after surgery as compared to untreated group.