ANALYSIS OF CD10 EXPRESSION IN THE EPITHELIAL LINING OF ODONTOGENIC CYSTS

  • Anam Ali Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Muhammad Asif Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Iram Ali The Hamdard College of Medicine & Dentistry (HCMD) Karachi Pakistan
  • Bismah Ahmad Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Muhammad Tahir Khadim Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Farhan Akhtar Armed Forces Institute of Pathology/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Muhammad Sajjad Abbasi CMH Multan Institute of Medical Sciences/National University of Medical Sciences (NUMS) Multan Pakistan
Keywords: CD10, Dentigerous cysts, Odontogenic keratocysts, Radicular cyst

Abstract

Objective: To investigate the expression of CD10 in the epithelium of Odontogenic keratocyst (OKC), Dentigerous and radicular cyst.

Study Design: Cross sectional analytical study.

Place and Duration of Study: Armed Forces Institute of Pathology, Rawalpindi, from Jan 2017 to Dec 2017.

Methodology: In this study, total of sixty cases were included with 20 of each Radicular cyst, Dentigerous cyst and Odontogenic keratocyst. Sections were stained with Haematoxylin and eosin (H&E) followed by Immuno-histochemistry (IHC) staining for CD10 antibody. SPSS version 20 was used to analyse the results. Expression of CD10 was evaluated.

Results: Out of total 60 cases, 41 (68.3%) were male and 19 (31.7%) were female patients with age ranges from 11 to 75 years. Mandible was reported in 38 (63.3%) of the cases followed by maxilla in 22 (36.7%) of the cases. In this study 7 (38%) cases of Odontogenic keratocysts showed negative epithelial CD10 expression as compared to Dentigerous cysts and Radicular cysts (45% & 25%). In Odontogenic keratocysts 9 (45%) of the cases showed basal layer of positivity of epithelium. While in dentigerous cyst 11 (55%) of the cases showed CD10 expression in the superficial layer of epithelium and the entire epithelium was positive in almost all 15 (75%) cases of radicular cyst. Intensity of staining was moderate in maximum number of the cases of Odontogenic keratocyst and radicular cyst (40% & 35%) as compared to dentigerous cyst (20%). There was a statistically significant association between odontogenic cysts and epithelial CD10 expression (p=0.001).

Conclusion: Majority of odontogenic cysts showed positivity for CD10 marker. Odontogenic keratocysts showed basal, DCs showed surface and RCs showed full thickness positivity for this marker.

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Published
2020-01-27
How to Cite
Ali, A., Asif, M., Ali, I., Ahmad, B., Khadim, M., Akhtar, F., & Abbasi, M. (2020). ANALYSIS OF CD10 EXPRESSION IN THE EPITHELIAL LINING OF ODONTOGENIC CYSTS. Pakistan Armed Forces Medical Journal, 70(Suppl-1), S10-14. Retrieved from https://pafmj.org/index.php/PAFMJ/article/view/3785
Section
Original Articles

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