AMELIORATIVE EFFECT OF BERBERIS VULGARIS FRUIT EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS

Authors

  • Lubna Faisal Liaquat National Hospital and Medical College, Karachi Pakistan
  • Zia -Ul- Islam Liaquat National Hospital and Medical College, Karachi Pakistan
  • Saima Athar Liaquat National Hospital and Medical College, Karachi Pakistan
  • Sadia Iqbal Dow University of Health Sciences, Karachi Pakistan
  • ```Fatima Rehman Bahria University Medical and Dental College, Karachi Pakistan
  • Syed Shariq Ullah Liaquat National Hospital and Medical College, Karachi Pakistan

DOI:

https://doi.org/10.51253/pafmj.v71i2.3505

Keywords:

Berberis vulgaris, Gentamicin, Kidney, Nephrotoxicity

Abstract

Objective: To observe the nephroprotective role of berberis vulgar is on renal parenchyma against microscopic and morphometric changes induced by Gentamicin in albino rats.

Study Design: Lab based experimental study.

Place and Duration of Study: It was conducted in Baqai Medical University in collaboration with department of Anatomy Liaquat National Hospital and Medical College, from Jan to Jul 2017.

Methodology: A total of 40 male adult albino rats were used in the study. Four groups were made. Each group contained 10 rats. Group A was a control group, group B received only berberis vulgaris fruit extract orally per day for 21 days, group C received Gentamicin 100 mg/kg/day intraperitonially daily for 21 days. Group D received Gentamicin 100 mg/kg/day intraperitonially along with berberis vulgaris fruit extract 100 mg/kg/day orally. Both kidneys were removed. H&E and PAS stains were used for observing histological alterations and protective role of berberis vulgaris fruit extract.

Results: Glomerulus and proximal convoluted tubules were observed histologically in all 4 groups. Microscopy of group B showed parameters nearly similar to control group. Microscopy of group C showed significant derangement in all parameters when compared with control group. Group C showed decrease glomerular, proximal convoluted tubular count was noted. Glomerular diameter increases and there was glomerular hypertrophy and tubular necrosis. Microscopy of group D showed significant improvement due to berberis vulgaris which restored normal renal architecture.

Conclusion: Berberis vulgaris has a nephroprotective effect and it can be used as a new medicine against nephrotoxic drugs like Gentamicin.

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Author Biographies

  • Zia -Ul- Islam, Liaquat National Hospital and Medical College, Karachi Pakistan

    Professor and Head department of Anatomy

  • Saima Athar, Liaquat National Hospital and Medical College, Karachi Pakistan

    Associate Professor department of Anatomy

  • Sadia Iqbal, Dow University of Health Sciences, Karachi Pakistan

    Assistant professor department of Anatomy

  • ```Fatima Rehman, Bahria University Medical and Dental College, Karachi Pakistan

    Lecturer department of Anatomy

  • Syed Shariq Ullah, Liaquat National Hospital and Medical College, Karachi Pakistan

    Lecturer department of Anatomy

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Published

30-04-2021

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Section

Original Articles

How to Cite

1.
Faisal L, Islam Z-U-, Athar S, Iqbal S, Rehman ```Fatima, Ullah SS. AMELIORATIVE EFFECT OF BERBERIS VULGARIS FRUIT EXTRACT AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN ALBINO RATS. Pak Armed Forces Med J [Internet]. 2021 Apr. 30 [cited 2024 Nov. 7];71(2):676-80. Available from: https://pafmj.org/PAFMJ/article/view/3505