ASSESSMENT OF SMOKE INDUCED GENOTOXICITY IN SPRAGUE DAWLEY RATS

  • Aneela Jamil Rawalpindi Medical College, Rawalpindi Pakistan
  • Amir Rashid Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Abdul Khaliq Naveed Islamic International Medical College/Riphah International University Islamabad Pakistan
Keywords: Cigarette smoking, Genotoxicity, Micronuclei

Abstract

Objective: To investigate the genotoxic effect of cigarette smoking in Sprague Dawley rats.

Study Design: Randomized experimental study.

Place and Duration of Study: Study was conducted at Army Medical College Rawalpindi, from Jan 2013 to Jul 2013.

Material and Methods: Seventy healthy Sprague Dawley rats were randomly divided into two groups at National Institute of Health (NIH). Group-I rats were not exposed while Group-II rats were exposed to cigarette smoke for 3 months. Cytokinesis Block Micronucleus Assay (CBMN) was done to determine genotoxicity. Data were subjected to statistical analysis using independent sample t-test.

Results: The mean frequency of micronuclei in peripheral blood lymphocytes of control and smoker groups was 3.06 ± 0.639 and 6.77 ± 0.731 respectively. The difference was not statistically significant (p>0.05).

Conclusion: The mean micronuclei frequency in peripheral blood lymphocytes of the smoke exposed rats was higher than that of the control rats however the difference was not significant.

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Published
2019-06-25
How to Cite
Jamil, A., Rashid, A., & Naveed, A. (2019). ASSESSMENT OF SMOKE INDUCED GENOTOXICITY IN SPRAGUE DAWLEY RATS. PAFMJ, 69(3), 577-81. Retrieved from https://pafmj.org/index.php/PAFMJ/article/view/3030
Section
Original Articles

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