EFFECT OF VISFATIN ON TESTOSTERONE LEVELS IN INSULIN RESISTANT DIABETIC MALE SPRAGUE DAWLEY RATS

Effect of Visfatin

Abstract

Objective: To study the effect of visfatin on testosterone levels in insulin resistant diabetic male Sprague Dawley rats.
Methods: Eighty male Sprague Dawley rats were randomly divided into four groups with twenty rats in each group. Group I served as control. Group II served as control insulin resistant diabetic. Rats in group III were made insulin resistant diabetics and were treated with visfatin. Rats in group IV were treated with visfatin and resistin after making them insulin resistant diabetics. Blood samples were taken and analyzed for serum testosterone, LH and FSH by ELISA.
Results: There was a significant rise in testosterone, FSH and LH levels in insulin resistant diabetic rats treated with visfatin. Co-administration of resistin did not result in an elevation of testosterone levels.
Conclusion: Visfatin increased testosterone levels and stimulated LH and FSH secretion from anterior pituitary.