TY - JOUR AU - Jamil, Aneela AU - Rashid, Amir AU - Naveed, Abdul Khaliq PY - 2019/06/25 Y2 - 2024/03/29 TI - ASSESSMENT OF SMOKE INDUCED GENOTOXICITY IN SPRAGUE DAWLEY RATS JF - Pakistan Armed Forces Medical Journal JA - PAFMJ VL - 69 IS - 3 SE - Original Articles DO - UR - https://pafmj.org/PAFMJ/article/view/3030 SP - 577-81 AB - <p><strong>Objective:</strong> To investigate the genotoxic effect of cigarette smoking in Sprague Dawley rats.</p><p><strong>Study Design:</strong> Randomized experimental study.</p><p><strong>Place and Duration of Study:</strong> Study was conducted at Army Medical College Rawalpindi, from Jan 2013 to Jul 2013.</p><p><strong>Material and Methods:</strong> Seventy healthy Sprague Dawley rats were randomly divided into two groups at National Institute of Health (NIH). Group-I rats were not exposed while Group-II rats were exposed to cigarette smoke for 3 months. Cytokinesis Block Micronucleus Assay (CBMN) was done to determine genotoxicity. Data were subjected to statistical analysis using independent sample t-test.</p><p><strong>Results:</strong> The mean frequency of micronuclei in peripheral blood lymphocytes of control and smoker groups was 3.06 ± 0.639 and 6.77 ± 0.731 respectively. The difference was not statistically significant (p&gt;0.05).</p><p><strong>Conclusion:</strong> The mean micronuclei frequency in peripheral blood lymphocytes of the smoke exposed rats was higher than that of the control rats however the difference was not significant.</p> ER -