@article{Yousuf_Ikram_Ikram_Nadeem_Hussain_Ali_2022, title={Chlamydia Trachomatis–A Cause of Infertility at Tertiary Care Hospital, Pakistan}, volume={72}, url={https://pafmj.org/PAFMJ/article/view/3166}, DOI={10.51253/pafmj.v72i4.3166}, abstractNote={<p><strong>Objective:</strong> To determine the association of Chlamydial infection with infertility in patients reporting to tertiary care set up for evaluation.</p> <p><strong>Study Design:</strong> Cross-sectional study.</p> <p><strong>Place and Duration of Study:</strong> Armed Forces Institute of Pathology, from Jun 2015 to Jun 2016.</p> <p><strong>Methodology:</strong> A total of 100 women with primary or secondary infertility were included. Endo cervical swabs were taken. PAP smear was performed. One drop of anti-chlamydial monoclonal antibody was added to each slide, incubated at 37oC for 10 minutes, and examined for Chlamydia trachomatis under the fluorescent microscope.</p> <p><strong>Results:</strong> The mean age was 29.878±3.48 years. 89.0% of the patients had primary infertility, and 11.0% had secondary infertility. The mean age of primary infertile patients was 29.98 ± 3.51 years, while the same was 29.00 ± 3.28 years among patients with secondary infertility. Chlamydial infection was detected positive by direct immunofluorescence (Elementary Bodies) in 6 patients with mean age of 27.17 ± 1.72 years and positive in 13 patients by polymerase chain reaction with mean age of 28.38 ± 1.85 years, having a significant p-value (<0.05).</p> <p><strong>Conclusion:</strong> There was an association between Chlamydia trachomatis infection and female infertility.</p>}, number={4}, journal={Pakistan Armed Forces Medical Journal}, author={Yousuf, Sana and Ikram, Aamir and Ikram, Sadia and Nadeem, Saman and Hussain, Syed Saddam and Ali, Syed Salman}, year={2022}, month={Aug.}, pages={1202–05} }