PROTECTIVE EFFECT OF SALBUTAMOL AND MONTELUKAST AGAINST INSULIN INDUCED AIRWAY HYPER-RESPONSIVENESS IN GUINEA PIG AIRWAYS IN VITRO

Insulin Induced Airway Hyper-Responsiveness

  • Mahjabeen Sharif Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Bushra Tayyaba Khan Army Medical College/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
  • Mubashir Sharif Armed Forces Institute of Dentistry/National University of Medical Sciences (NUMS) Rawalpindi Pakistan
Keywords: Inhaled insulin, Oscillograph, Montelukast, Salbutamol, Tracheal muscle

Abstract

Objective: To evaluate and compare the protective effects of salbutamol and montelukast in amelioration of insulin induced airway hyper-reactivity on isolated tracheal smooth muscle of guinea pig in vitro.
Study Design: Laboratory based randomized control trials (Experimental study).
Place and Duration of Study: Pharmacology Department and Centre for Research in Experimental and Applied Medicine (CREAM) Army Medical College Rawalpindi from January 2012 to September 2012.
Material and Methods: Effects of variable doses of insulin (10-7-10-3 M) and insulin pretreated with salbutamol (10-6 M) and montelukast (10-5 M) were observed on airways of guinea pig in vitro by constructing cumulative dose response curves. Four Channel Oscillograph and Transducer were used to record the tracheal smooth muscle contractions.
Results: Insulin induced a dose dependent reversible contraction of isolated tracheal muscle of guinea pig. The maximum response of insulin and insulin pretreated with salbutamol and montelukast were 35 ± 1.13 mm, 14.55 ± 0.62 mm and 34.5 ± 1.024 mm respectively. So salbutamol significantly inhibited the contractile response of insulin while montelukast failed to counteract the airway hyper-reactivity induced by insulin.
Conclusion: Salbutamol significantly inhibited the contractile response of insulin, while montelukast did not produce statistically significant effect on insulin induced airway hyper-reactivity. So it is suggested that pretreatment of inhaled insulin with salbutamol may be beneficial in inhibiting the broncho-constriction induced by insulin.

 

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Published
2016-10-31
How to Cite
Sharif, M., Khan, B., & Sharif, M. (2016). PROTECTIVE EFFECT OF SALBUTAMOL AND MONTELUKAST AGAINST INSULIN INDUCED AIRWAY HYPER-RESPONSIVENESS IN GUINEA PIG AIRWAYS IN VITRO. Pakistan Armed Forces Medical Journal, 66(5), 726-30. Retrieved from https://pafmj.org/index.php/PAFMJ/article/view/838
Section
Original Articles