EFFECT OF ACETYLSALICYLIC ACID ON SPERMATOGENESIS IN ADULT ALBINO MICE

Effect of Acetylsalicylic Acid on Spermatogenesis

Abstract

Objective: To Observe the histological and morphological effects of acetylsalicylic acid (ASA) on spermatogenesis in male albino mice.

Study Design: Laboratory based randomized controlled trial.

Place and Duration of Study: Department of Anatomy University of Health Sciences, Lahore from Apr, 2012 to Dec, 2012.

Material and Methods: Thirty nine male albino mice, 6-8 weeks old weighing 30 ± 5 gm, were used; these were randomly divided into three groups having thirteen mice in each using random numbers table. Group A served as a control and was given distilled water orally via oral gavage 10 ml per kg for 30 days. Group B was given acetylsalicylic acid 100 mg/kg dissolved in 10 ml distilled orally for a period of 30 days. Group C was given acetylsalicylic acid 25 mg/kg dissolved in 2.5 ml distilled orally for a period of 30 days. Animals were sacrificed 24 hours after the last dose and the testes were removed, fixed in Bouin’s fixative for 48 hours. Five microns thick sections of processed tissue were stained with H&E and PAS for calculation of Johnsen score and diameter of seminiferous tubules. Serum testosterone level was measured by testosterone enzyme immunoassay test kits.

Results: Microscopic examination demonstrated that ASA treatment lead to statistically significant increase in the mean Johnsen score and mean diameter of seminiferous tubules.

Conclusion: It was concluded from the current study that ASA treatment enhances spermatogenesis.