EFFECTS OF MICROWAVE OVEN EXPOSED DIET ON SPERMATOGENESIS IN TESTICULAR TISSUE OF MICE AND COMPARATIVE EFFECTS OF MENTHA PIPERITA AND MELATONIN

Microwave Exposure & Spermatogenesis in Mice

Abstract

Objective: To observe the effects of microwave oven exposed diet on spermatogenesis in the testis of mice and comparative effects of Mentha piperita and melatonin.
Study Design: Laboratory based randomized controlled trial.
Place and Duration of Study: Anatomy Department, Army Medical College Rawalpindi, in collaboration with National Institute of Health (NIH), Islamabad, from Apr 2015 to May 2015.
Material and Method: Study comprised of 32 adult male mice (BALBc strain) weighing 25-30 gms. Selection criteria based on non-probability (purposive) simple random sampling. Mice were divided into four equal groups of 8 mice each. Group 1, taken as control, was given standard diet 5-10gm/animal/day daily for four weeks. Group 2 was given 5-10 gm/animal/day of microwave oven exposed mice pellets for four weeks. Group 3 received Mentha piperita leaf extract (1g/kg b.wt./day) along with microwave oven exposed mice pellets (5-10gm/animal/day) for 4 weeks and group 4 received oral dosage of melatonin 12mg/kg/day along with microwave oven exposed mice pellets (5-10gm/animal/day) for 4 weeks. After four weeks animals were dissected. The shape, color and any abnormal finding of the testis were observed. Testis were processed, embedded and stained for histological study. Spermatogenesis was assessed by the Johnsons scoring. SPSS 21 was used for statistical analysis. Chi square test was applied for intergroup comparison.
Results: Spermatogenesis was suppressed and Johnsons score was decreased from normal spermatogenesis (10) to (6-8) in the experimental group 2 and was more improved in the Mentha piperita treated group as compare to the melatonin.
Conclusion: Microwave oven exposed mice pellets suppressed spermatogenesis and Mentha piperita had better ameliorative effects than melatonin on the testis of mice.
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