PAKISTAN ARMED FORCES
MEDICAL JOURNAL (Category Y)

A Journal of Army Medical & Dental Corps

Being published since 1956

ISSN (online) 2411-8842
ISSN (print) 0030-9648

VOL 65, No. 2, APRIL 2015

FREQUENCY OF ENTEROCOCCUS FAECALIS IN SALIVA AND ROOT CANALS WITH TREATMENT FAILURE

Imran Khan*, Tabinda Shan, Manzoor Ahmed Manzoor

Abstract

Objective: To compare the frequency of E. faecalis in the saliva and root canals of teeth associated with apical periodontitis due to endodontic treatment failure in the same patient.
Study Design: Cross-sectional comparative study.
Place and Duration of Study: Samples were collected from Operative Dentistry Department, AFID, while laboratory processing was done at AFIP, Rawalpindi. Study duration was one year.
Patients and Methods: Fifty patients, both males and females with failed endodontic treatment were selected. Saliva and root canal samples were collected from each patient, inoculated on MacKonkey agar plate and incubated at 35-370C for 48 hrs. E. faecalis colonies were identified by colony morphology, gramstain, catalase, bile asculin test, arabinose fermentation and growth in 6% NaCl nutrient broth.
Results: The frequency of E. faecalis in saliva was 34% and in root canal it was 58%. Frequency between the presence of E. faecalis in root canals and saliva was found to be statistically different (p = 0.001).
Conclusion: The presence of E. faecalis in root canal was not associated with their presence in saliva.

Keywords : Apical periodontitis, Endodontic treatment failure, Enterococcus faecalis.



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