Objective: To investigate the effect of cigarette smoking on interleukin-6 in Sprague Dawley rats.
Study Design: Randomized controlled study.
Place and Duration of Study: Study was conducted at Army Medical College Rawalpindi, from Jan to Apr 2015.
Material and Methods: Seventy healthy male Sprague Dawley rats were randomly divided into two groups at National Institute of Health (NIH). Group-I rats were not exposed while group-II rats were exposed to cigarette smoke for 3 months. Interleukin-6 (IL-6) was determined using commercially available enzyme linked immunosorbent assay (ELISA) kit. Data was analyzed using t-test.
Results: Higher levels of IL-6 were observed in exposed than in non-exposed. The mean of IL-6 levels of control and smoker groups was 40.84 ± 9.09 and 49.48 ± 19.69 respectively. The difference was statistically significant (p<0.05).
Conclusion: Positive association was found between cigarette smoking and IL-6. This shows that IL-6 may be used as a biochemical marker to assess inflammatory damage in smoke related diseases.
Keywords : Cigarette, IL-6, Inflammation, Interleukin, Smoking.