PAKISTAN ARMED FORCES
MEDICAL JOURNAL (Category Y)

A Journal of Army Medical & Dental Corps

Being published since 1956

ISSN (online) 2411-8842
ISSN (print) 0030-9648

VOL 68, No. 3, JUNE 2018

EFFECT OF SMOKING ON INTERLEUKIN-6 IN SPRAGUE DAWLEY RATS

Aneela Jamil, Amir Rashid*, Abdul Khaliq Naveed**

Abstract

 

Objective: To investigate the effect of cigarette smoking on interleukin-6 in Sprague Dawley rats.
Study Design: Randomized controlled study.
Place and Duration of Study: Study was conducted at Army Medical College Rawalpindi, from Jan to Apr 2015.
Material and Methods: Seventy healthy male Sprague Dawley rats were randomly divided into two groups at National Institute of Health (NIH). Group-I rats were not exposed while group-II rats were exposed to cigarette smoke for 3 months. Interleukin-6 (IL-6) was determined using commercially available enzyme linked immunosorbent assay (ELISA) kit. Data was analyzed using t-test.
Results: Higher levels of IL-6 were observed in exposed than in non-exposed. The mean of IL-6 levels of control and smoker groups was 40.84 ± 9.09 and 49.48 ± 19.69 respectively. The difference was statistically significant    (p<0.05).
Conclusion: Positive association was found between cigarette smoking and IL-6. This shows that IL-6 may be used as a biochemical marker to assess inflammatory damage in smoke related diseases.

Keywords : Cigarette, IL-6, Inflammation, Interleukin, Smoking.



Creative Commons License
This is an Open Access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.